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- W3036694753 abstract "Pregnant rats (day 13) received 10mg/kg of Busulfan i.p. in order to deplete of germ cell the testes of the new born rats. The seminiferous tubules of their off spring from postnatal age 1 day up to day 35 were examined with TEM after fixation plus intercellular tracers, and with freeze-fracture techniques. During this period, the inter-Sertoli tight junctions of controls increase both in numbers and in length. Between days 10 and 13 the seminiferous cords have numerous preleptotene and leptotene spermatocytes (Fig.1) surrounded by tracer. The inter-Sertoli junctions are tortuous and predominantly perpendicular to the basal lamina. Between ages 13 and 20 days the seminiferous epithelium reaches zygotene-pachytene stages (fig.2) identified by the presence of synaptonemal complexes. The tracer is stopped at the inter-Sertoli junctions at this stage, whereas it still permeates tubules displaying preleptotene and leptotene spermatocytes. Freeze-fracture shows that the orientation of inter-Sertoli junctions has changed to parallel, both to each other and to the basal lamina (fig.3). In the Busulfan treated rats, the tubules continue having, up to postnatal day 30, only Sertoli cells and scanty spermatogonia." @default.
- W3036694753 created "2020-06-25" @default.
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- W3036694753 date "1990-08-12" @default.
- W3036694753 modified "2023-09-26" @default.
- W3036694753 title "Correlation between blood-testis barrier development and onset of the first spermatogenic wave in normal and busulfan-treated rats" @default.
- W3036694753 cites W2082192145 @default.
- W3036694753 doi "https://doi.org/10.1017/s0424820100157930" @default.
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