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- W3036697742 abstract "Scanning electron microscopic immunohistochemical observations have been performed mainly using antibodies labeled by either virus particle or colloidal gold. In order to observe immunoreactive sites using these markers, however, high magnification is needed, even when the backscattered electron image (BEI) mode is utilized. Using alkaline phosphatase (ALPase)-labeled antibodies, lead phosphate can be deposited on immunoreactive sites and is detectable as strong signals on the BEI. Recent progress in computer technology enabled us to superimpose two or more images using different colors and gray levels. In this study, our attempt was made to detect the localization of prolactin cells in the pituitary gland and of laminin in the kidney using ALPase-labeled antibodies, by superimposing immunoreactive sites indicated by the BEI over their surface structures shown by the secondary electron image (SEI). Anterior pituitary glands obtained from female mice and kidneys from male mice were fixed by perfusion with 4% paraformaldehyde in 0.1M sodium phosphate buffer (SPB). After rinsing with 0.1M SPB, the fixed specimens were transferred into 50% dimethyl sulfoxide, frozen in liquid nitrogen and fractured, and rinsed with 0.1M SPB. The kidneys were pretreated with 0.1% pepsin in 0.01N HCl in order to have immunoreactivity against laminin restored." @default.
- W3036697742 created "2020-06-25" @default.
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- W3036697742 date "1990-08-12" @default.
- W3036697742 modified "2023-10-14" @default.
- W3036697742 title "SEM immunohistochemistry using alkaline phosphatase-labeled antibody" @default.
- W3036697742 cites W2003642478 @default.
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- W3036697742 doi "https://doi.org/10.1017/s0424820100162016" @default.
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