Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036699953> ?p ?o ?g. }
- W3036699953 abstract "Abstract An orthogonal aminoacyl-tRNA synthetase/tRNA pair is a crucial prerequisite for site-specific incorporation of unnatural amino acids. Due to its high codon suppression efficiency and full orthogonality, the pyrrolysyl-tRNA synthetase/pyrrolysyl-tRNA pair is currently the ideal system for genetic code expansion in both eukaryotes and prokaryotes. There is a pressing need to discover or engineer other fully orthogonal translation systems. Here, through rational chimera design by transplanting the key orthogonal components from the pyrrolysine system, we create multiple chimeric tRNA synthetase/chimeric tRNA pairs, including chimera histidine, phenylalanine, and alanine systems. We further show that these engineered chimeric systems are orthogonal and highly efficient with comparable flexibility to the pyrrolysine system. Besides, the chimera phenylalanine system can incorporate a group of phenylalanine, tyrosine, and tryptophan analogues efficiently in both E. coli and mammalian cells. These aromatic amino acids analogous exhibit unique properties and characteristics, including fluorescence, post-translation modification." @default.
- W3036699953 created "2020-06-25" @default.
- W3036699953 creator A5023513323 @default.
- W3036699953 creator A5027013375 @default.
- W3036699953 creator A5046881277 @default.
- W3036699953 creator A5049692788 @default.
- W3036699953 creator A5050577040 @default.
- W3036699953 creator A5051704098 @default.
- W3036699953 creator A5076026075 @default.
- W3036699953 creator A5079061656 @default.
- W3036699953 date "2020-06-22" @default.
- W3036699953 modified "2023-10-12" @default.
- W3036699953 title "Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion" @default.
- W3036699953 cites W1965897796 @default.
- W3036699953 cites W1966087112 @default.
- W3036699953 cites W1967666740 @default.
- W3036699953 cites W1969239697 @default.
- W3036699953 cites W1970743827 @default.
- W3036699953 cites W1973268713 @default.
- W3036699953 cites W1976327539 @default.
- W3036699953 cites W1982826901 @default.
- W3036699953 cites W1985650731 @default.
- W3036699953 cites W1986735811 @default.
- W3036699953 cites W1989636060 @default.
- W3036699953 cites W1992502609 @default.
- W3036699953 cites W1992932889 @default.
- W3036699953 cites W2029672674 @default.
- W3036699953 cites W2034500175 @default.
- W3036699953 cites W2039519304 @default.
- W3036699953 cites W2045803636 @default.
- W3036699953 cites W2052858356 @default.
- W3036699953 cites W2061377697 @default.
- W3036699953 cites W2064942594 @default.
- W3036699953 cites W2069182434 @default.
- W3036699953 cites W2072163663 @default.
- W3036699953 cites W2074244626 @default.
- W3036699953 cites W2076992251 @default.
- W3036699953 cites W2077785805 @default.
- W3036699953 cites W2081262930 @default.
- W3036699953 cites W2092118306 @default.
- W3036699953 cites W2095370301 @default.
- W3036699953 cites W2097737716 @default.
- W3036699953 cites W2100475836 @default.
- W3036699953 cites W2107167859 @default.
- W3036699953 cites W2107511608 @default.
- W3036699953 cites W2111158627 @default.
- W3036699953 cites W2115862579 @default.
- W3036699953 cites W2126183505 @default.
- W3036699953 cites W2128057497 @default.
- W3036699953 cites W2133450786 @default.
- W3036699953 cites W2148123458 @default.
- W3036699953 cites W2157680389 @default.
- W3036699953 cites W2168635905 @default.
- W3036699953 cites W2231352968 @default.
- W3036699953 cites W2272264121 @default.
- W3036699953 cites W2283105825 @default.
- W3036699953 cites W2312277739 @default.
- W3036699953 cites W2312359800 @default.
- W3036699953 cites W2344294224 @default.
- W3036699953 cites W2557567892 @default.
- W3036699953 cites W2573666190 @default.
- W3036699953 cites W2588345894 @default.
- W3036699953 cites W2731987565 @default.
- W3036699953 cites W2759669407 @default.
- W3036699953 cites W2763278454 @default.
- W3036699953 cites W2766423195 @default.
- W3036699953 cites W2767046452 @default.
- W3036699953 cites W2773265882 @default.
- W3036699953 cites W2777700910 @default.
- W3036699953 cites W2788298310 @default.
- W3036699953 cites W2791241064 @default.
- W3036699953 cites W2803722031 @default.
- W3036699953 cites W2808770696 @default.
- W3036699953 cites W2809896272 @default.
- W3036699953 cites W2884127564 @default.
- W3036699953 cites W2884151060 @default.
- W3036699953 cites W2888368935 @default.
- W3036699953 cites W2899760200 @default.
- W3036699953 cites W2944460251 @default.
- W3036699953 cites W4211164196 @default.
- W3036699953 cites W4231832388 @default.
- W3036699953 doi "https://doi.org/10.1038/s41467-020-16898-y" @default.
- W3036699953 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7308279" @default.
- W3036699953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32572025" @default.
- W3036699953 hasPublicationYear "2020" @default.
- W3036699953 type Work @default.
- W3036699953 sameAs 3036699953 @default.
- W3036699953 citedByCount "34" @default.
- W3036699953 countsByYear W30366999532020 @default.
- W3036699953 countsByYear W30366999532021 @default.
- W3036699953 countsByYear W30366999532022 @default.
- W3036699953 countsByYear W30366999532023 @default.
- W3036699953 crossrefType "journal-article" @default.
- W3036699953 hasAuthorship W3036699953A5023513323 @default.
- W3036699953 hasAuthorship W3036699953A5027013375 @default.
- W3036699953 hasAuthorship W3036699953A5046881277 @default.
- W3036699953 hasAuthorship W3036699953A5049692788 @default.
- W3036699953 hasAuthorship W3036699953A5050577040 @default.
- W3036699953 hasAuthorship W3036699953A5051704098 @default.
- W3036699953 hasAuthorship W3036699953A5076026075 @default.