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- W3036701188 abstract "Coagulase-negative Staphylococci have been implicated in late onset infection of breast prosthetic biomaterials used for augmentation and reconstruction. These prosthesis consist of either gel- or saline-filled smooth silicone rubber or textured polyurethane foam. The morphology of adhesion of staphylococci to the prosthesis is important in understanding their potential role in latent infection. Staphylococcus epidermidis rp62, a polysaccaride mucin producing strain, and Staphylococcus aureus strain 25923 are test organisms. A variety of fixative procedures employing en bloc reagents are compared in both SEM and TEM. Ruthenium red and alcian blue , uranyl acetate and ruthenium red with lysine have been used to preserve extracellular capsular/slime material in several bacterial species. Specimens of prosthetic material are incubated with cells for 18 hrs at 35°C in tryptic soy broth. For SEM, specimens are fixed in 2.5% glutaraldehyde (GA) for 2 hrs and post-fixed in 1% OsO 4 for 2.5 hrs. This is followed by a graded ethanol series, critical point drying with CO 2 and coating with gold/palladium." @default.
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- W3036701188 date "1990-08-12" @default.
- W3036701188 modified "2023-09-27" @default.
- W3036701188 title "SEM and TEM evaluation of bacterial adhesion morphology to breast prosthetic biomaterials" @default.
- W3036701188 cites W1972228119 @default.
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- W3036701188 doi "https://doi.org/10.1017/s0424820100161783" @default.
- W3036701188 hasPublicationYear "1990" @default.
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