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- W3036787647 abstract "The mature bacteriophage λ head contains two major proteins: gene products E and D (gpE and gpD). These proteins are arranged in a composite (gpE/gpD ratio = 1/1) T=7 icosahedral surface lattice. In previous studies using tubular head related forms, “polyheads”, we had proposed models for head maturation and structure. But with the methods used, it was not possible to localize unambiguously the position of the gpD clusters added during the last steps of the head maturation. New experiments are performed to choose between the two different models previously proposed. In a first step we have analysed with optical filtration some structures obtained after self-assembly of guanidine hydrochloride dissociated heads. One sided preferentially stained polyhead (Fig. 1) or monolayer (sheets) (Fig. 2) show the triangular shape clusters which could be the gpD trimers. We were able to get some improvement of the resolution of normal polyhead images by removal of associated debris with a mild treatment of citraconyl anhydride (10 mg/mg of polyhead) before preparing the specimen for the conventional electron microscope (Fig. 3) or the scanning transmission electron microscope (Fig. 4)." @default.
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- W3036787647 date "1978-08-01" @default.
- W3036787647 modified "2023-10-18" @default.
- W3036787647 title "Surface structure of the bacteriophage λ head: location of the gpD" @default.
- W3036787647 cites W2010309573 @default.
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- W3036787647 doi "https://doi.org/10.1017/s0424820100082790" @default.
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