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- W3036791090 abstract "Electron-lucent regions in the junctional sarcoplasmic reticulum (JSR) were first observed in frog skeletal muscle treated with ruthenium red and other exotic cations. Next, they were pointed out in Epon sections of freeze-substituted muscle following quick-freezing both in the presence and absence of cryoartifacts. Whereas their nature and function remain obscure, the so-called “core cylinders” (CCs) are now considered invariant components of the normal anatomy of frog skeletal muscle JSR. Following quick-freezing, part of the single intact frog skeletal muscle fiber was freeze-substituted, embedded in Epon and sectioned (Fig. 1). Cryosections were cut from an immediately adjacent fiber region with a diamond knife specially mounted for that purpose with two free edges (courtesy Diatome-US), on a Reichert Ultracut FC-4D at -150°C, put on the carbon film on 100 mesh nickel grids, freeze-dried in a Balzers FDU 010 fitted with a turbomolecular pump (vac 10 -6 torr) against a liquid N 2 trap (geometry 14 mm) for 15 hrs with 3 temperature steps, each 3 hrs apart, at -100, -85, and -50°C, respectively (Fig. 2)." @default.
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- W3036791090 date "1987-08-01" @default.
- W3036791090 modified "2023-09-27" @default.
- W3036791090 title "The core cylinder of the JSR of frog skeletal muscle in cryosections" @default.
- W3036791090 cites W2036701273 @default.
- W3036791090 doi "https://doi.org/10.1017/s042482010012686x" @default.
- W3036791090 hasPublicationYear "1987" @default.
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