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- W3036791434 abstract "Viroids are uncoated infectious RNA molecules pathogenic to certain higher plants. The molecular structure of viroids is characterised by single-strandedness, circularity and partial complementarity within the chain of about 350 nucleotides. In the native state the viroid molecule exists as a highly base-paired rod-like structure. Fig. 1 shows a dark-field micrograph of cucumber pale fruit viroid (CPFV) spread with cytochrome c and positively stained with uranyl salts. Under our conditions rods measure 37. 5 nm ± 2. 5 nm in length. They appear slightly flexible. Specific details are not recognized. Using benzyldimethylalkylammonium chloride (BAC) as the film forming surfactant most of the molecules appear as dumb-bell shaped rods with terminal knots (Fig. 2). Length determination led to the mean value of 33 nm ± 3 nm. The variety of structures previously described was further investigated to assess that they reflect different conformational states of only one species of molecules." @default.
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- W3036791434 date "1978-08-01" @default.
- W3036791434 modified "2023-10-18" @default.
- W3036791434 title "conformational studies of covalently closed circular viroid RNA" @default.
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- W3036791434 doi "https://doi.org/10.1017/s0424820100082819" @default.
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