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- W3036804426 abstract "Cell volume changes and regulation are thought to be important in the physiology and pathology of neurons. Osmotic challenges alter a number of physiologic parameters including electrical properties, chemosensitivity, and Na pump activity. Thus, a good method for correlating images of the living cell's surface with electrophysiologic measures is needed, and we are exploring the use of confocal light microscopy for imaging neurons in culture. Isolated neurons from ganglia of Aplvsia California were plated on polylysine coated coverslips in L15 media with 20% hemolymph at room temperature. Cells stained with “Dil” were imaged with a Bio—Rad MRC—600 and an Olympus BH—2. Images were displayed using Bio—Rad's maximum projection procedure, or Vital Images’ Voxel View software run on an IBM RISC 6000 Powerstation 320. Electrophysiologic properties were assessed after imaging." @default.
- W3036804426 created "2020-06-25" @default.
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- W3036804426 date "1991-08-01" @default.
- W3036804426 modified "2023-09-25" @default.
- W3036804426 title "Confocal light microscopy and electrophysiology of neurons in culture" @default.
- W3036804426 cites W1998524706 @default.
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- W3036804426 doi "https://doi.org/10.1017/s0424820100086301" @default.
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