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- W3036804940 abstract "The resolution of the SEM has progressed markedly by means of an “in-lens” type field emission (FE) SEM. However, its performance at low beam energy, where we could expect an improved resolving power to visualize the detail of the surface of the specimens, was insufficient. Therefore an improved ultrahigh resolution (UHR) FESEM, the S-900H, was developed jointly with Hitachi to give better resolution at low voltages (LV, below 5 kV) by improving the objective pole piece. This instrument, which we called model S-900 LV, allows visualization of an about 1-nm object at 2.5 kV and x 30,000. The instrument made it possible to observe uncoated biological specimens at a magnification of 200,000 in the LV from 2 to 3 kV. The present study was intended to elucidate the u1trastructure of a fission yeast protoplast in the early stage of its regeneration, especially the formation of glucan fibrils from secreted small particles." @default.
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- W3036804940 date "1990-08-12" @default.
- W3036804940 modified "2023-10-16" @default.
- W3036804940 title "Initial formation of glucan fibrils of the reverting protoplast in fission yeast, elucidated with a newly developed ultrahigh-resolution, low-voltage scanning electron microscope" @default.
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- W3036804940 doi "https://doi.org/10.1017/s0424820100157619" @default.
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