Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036850265> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3036850265 abstract "Abstract Temporal modulation of multiple genes underlies sophisticated biological phenomena. However, there are few scalable and generalizable gene circuit architectures for the programming of sequential genetic perturbations. We describe a modular recombinase-based gene circuit architecture, comprising tandem gene perturbation cassettes (GPCs), that enables the sequential expression of multiple genes in a defined temporal order by alternating treatment with just two orthogonal ligands. We used tandem GPCs to sequentially express single-guide RNAs to encode transcriptional cascades and trigger the sequential accumulation of mutations. We built an all-in-one gene circuit that sequentially edits genomic loci, synchronizes cells at a specific stage within a gene expression cascade, and deletes itself for safety. Tandem GPCs offer a multi-tiered cellular programming tool for modeling multi-stage genetic changes, such as tumorigenesis and cellular differentiation." @default.
- W3036850265 created "2020-06-25" @default.
- W3036850265 creator A5015981980 @default.
- W3036850265 creator A5023856282 @default.
- W3036850265 creator A5045782161 @default.
- W3036850265 creator A5060356548 @default.
- W3036850265 date "2020-06-20" @default.
- W3036850265 modified "2023-09-25" @default.
- W3036850265 title "Scalable recombinase-based gene expression cascades" @default.
- W3036850265 cites W1931339766 @default.
- W3036850265 cites W1992783297 @default.
- W3036850265 cites W1998947396 @default.
- W3036850265 cites W2009283961 @default.
- W3036850265 cites W2028543816 @default.
- W3036850265 cites W2068962163 @default.
- W3036850265 cites W2073405971 @default.
- W3036850265 cites W2074700940 @default.
- W3036850265 cites W2088245621 @default.
- W3036850265 cites W2097173606 @default.
- W3036850265 cites W2100249996 @default.
- W3036850265 cites W2141172277 @default.
- W3036850265 cites W2146142395 @default.
- W3036850265 cites W2158243563 @default.
- W3036850265 cites W2163173465 @default.
- W3036850265 cites W2209402024 @default.
- W3036850265 cites W2219121760 @default.
- W3036850265 cites W2427010553 @default.
- W3036850265 cites W2464464964 @default.
- W3036850265 cites W2501736566 @default.
- W3036850265 cites W2509763102 @default.
- W3036850265 cites W2603648333 @default.
- W3036850265 cites W2900498998 @default.
- W3036850265 cites W2915459742 @default.
- W3036850265 cites W2953135660 @default.
- W3036850265 cites W2982082283 @default.
- W3036850265 cites W2992097433 @default.
- W3036850265 cites W2992368546 @default.
- W3036850265 doi "https://doi.org/10.1101/2020.06.20.161430" @default.
- W3036850265 hasPublicationYear "2020" @default.
- W3036850265 type Work @default.
- W3036850265 sameAs 3036850265 @default.
- W3036850265 citedByCount "0" @default.
- W3036850265 crossrefType "posted-content" @default.
- W3036850265 hasAuthorship W3036850265A5015981980 @default.
- W3036850265 hasAuthorship W3036850265A5023856282 @default.
- W3036850265 hasAuthorship W3036850265A5045782161 @default.
- W3036850265 hasAuthorship W3036850265A5060356548 @default.
- W3036850265 hasBestOaLocation W30368502651 @default.
- W3036850265 hasConcept C102230213 @default.
- W3036850265 hasConcept C102414288 @default.
- W3036850265 hasConcept C104317684 @default.
- W3036850265 hasConcept C125929170 @default.
- W3036850265 hasConcept C141035611 @default.
- W3036850265 hasConcept C141231307 @default.
- W3036850265 hasConcept C150194340 @default.
- W3036850265 hasConcept C156695909 @default.
- W3036850265 hasConcept C165864922 @default.
- W3036850265 hasConcept C27149982 @default.
- W3036850265 hasConcept C41008148 @default.
- W3036850265 hasConcept C48044578 @default.
- W3036850265 hasConcept C54355233 @default.
- W3036850265 hasConcept C66746571 @default.
- W3036850265 hasConcept C70721500 @default.
- W3036850265 hasConcept C77088390 @default.
- W3036850265 hasConcept C86803240 @default.
- W3036850265 hasConceptScore W3036850265C102230213 @default.
- W3036850265 hasConceptScore W3036850265C102414288 @default.
- W3036850265 hasConceptScore W3036850265C104317684 @default.
- W3036850265 hasConceptScore W3036850265C125929170 @default.
- W3036850265 hasConceptScore W3036850265C141035611 @default.
- W3036850265 hasConceptScore W3036850265C141231307 @default.
- W3036850265 hasConceptScore W3036850265C150194340 @default.
- W3036850265 hasConceptScore W3036850265C156695909 @default.
- W3036850265 hasConceptScore W3036850265C165864922 @default.
- W3036850265 hasConceptScore W3036850265C27149982 @default.
- W3036850265 hasConceptScore W3036850265C41008148 @default.
- W3036850265 hasConceptScore W3036850265C48044578 @default.
- W3036850265 hasConceptScore W3036850265C54355233 @default.
- W3036850265 hasConceptScore W3036850265C66746571 @default.
- W3036850265 hasConceptScore W3036850265C70721500 @default.
- W3036850265 hasConceptScore W3036850265C77088390 @default.
- W3036850265 hasConceptScore W3036850265C86803240 @default.
- W3036850265 hasLocation W30368502651 @default.
- W3036850265 hasLocation W30368502652 @default.
- W3036850265 hasOpenAccess W3036850265 @default.
- W3036850265 hasPrimaryLocation W30368502651 @default.
- W3036850265 hasRelatedWork W10314934 @default.
- W3036850265 hasRelatedWork W11110208 @default.
- W3036850265 hasRelatedWork W365566 @default.
- W3036850265 hasRelatedWork W4134988 @default.
- W3036850265 hasRelatedWork W5061396 @default.
- W3036850265 hasRelatedWork W5794924 @default.
- W3036850265 hasRelatedWork W7090190 @default.
- W3036850265 hasRelatedWork W7863678 @default.
- W3036850265 hasRelatedWork W7870589 @default.
- W3036850265 hasRelatedWork W9955048 @default.
- W3036850265 isParatext "false" @default.
- W3036850265 isRetracted "false" @default.
- W3036850265 magId "3036850265" @default.
- W3036850265 workType "article" @default.