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- W3036862815 abstract "In cryofixation of biological samples it is typically desirable to minimize or avoid ice crystals in the sample by one of several rapid cooling techniques. Using these techniques freezing rates of 1,000 -10,000 °C/s are commonly achieved at a cooling boundary. At these rates, cellular dehydration is typically absent, and intracellular ice crystals are so small as to be considered negligible within an “ice-free” zone typically ranging 5-25 μm into the sample from the cooling boundary. However, during medical applications of freezing including cryopreservation as well as cryosurgery, the rate of freezing is orders of magnitude less than the idealized freezing procedures used in cryofixation. During slower freezing at rates between 1 - 100 °C/min, significant water transport between the cells and the extracellular/vascular space of a tissue can occur. This dehydration affects how and where the ice will form in the tissue which in turn can influence the post-freeze viability and therefore the overall success of a medical freezing treatment. The purpose of this work is to obtain a more quantitative understanding and prediction of the biophysics of water transport in liver tissue during freezing." @default.
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- W3036862815 date "1995-08-13" @default.
- W3036862815 modified "2023-09-26" @default.
- W3036862815 title "A new microscopy method to assess mass transfer during freezing in rat liver tissue slices" @default.
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- W3036862815 doi "https://doi.org/10.1017/s042482010014169x" @default.
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