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• W3036884032 endingPage "112394" @default.
• W3036884032 startingPage "112394" @default.
• W3036884032 abstract "This paper reports a quantitative and sensitive one-step point-of-care testing (POCT) chip built on a perfluorinated substrate patterned with polydopamine microspots array. The capture antibody was covalently immobilized on the polydopamine microspots, while the fluorescently labelled detection antibody was physically adsorbed on the perfluorinated surface. The POCT chip allowed one-step sandwich immunoassay and was able to directly detect the analytes from the whole blood without sample preprocessing. By further taking advantages of the strong fluorescence quenching ability of the polydopamine, the blocking-free substrate was able to achieve similar performance in detecting and quantifying the protein biomarkers as the substrate with the blocking treatment. The blocking-free strategy not only made the fabrication of the chip simple and convenient, but also improved the chip's sensitivity for biomarker quantification. Finally, we demonstrated that the self-contained POCT platform maintained the performance for one-step immunoassay even after long-term storage. With the POCT platform, we are one step closer to a sample-in-answer-out diagnostic system." @default.
• W3036884032 created "2020-06-25" @default.
• W3036884032 creator A5009411759 @default.
• W3036884032 creator A5069047152 @default.
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• W3036884032 date "2020-10-01" @default.
• W3036884032 modified "2023-10-16" @default.
• W3036884032 title "Blocking-free and self-contained immunoassay platform for one-step point-of-care testing" @default.
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• W3036884032 doi "https://doi.org/10.1016/j.bios.2020.112394" @default.
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