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- W3036967533 abstract "Scanning tunneling microscopy (STM) enables remarkable visualization of surface topography with a vertical resolution of 0.2 Å and horizontal resolution of 2-3 Å. The possibility of visualizing nucleic acids at this high resolution has major significance, since the binding of proteins and other aspects of gene regulation depend on structural variations at the Å level. Conventional EM has the advantage over fiber diffraction or solution techniques that local structure of individual molecules can be seen, rather than averaging over entire molecules and populations of molecules. An added advantage of STM is that contrast is achieved by detection of sample height and work function variations, rather than by chemical treatments such as shadowing or staining. We have used STM to measure the helical pitch, half-period oscillations interpreted as alternating major and minor grooves, and molecular diameters of poly(rA)-poly(rU) and DNA. Average pitches are measured by two-dimensional Fourier transforms and by topographic profile peak count vs." @default.
- W3036967533 created "2020-06-25" @default.
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- W3036967533 date "1990-08-12" @default.
- W3036967533 modified "2023-09-27" @default.
- W3036967533 title "STM of nucleic acids" @default.
- W3036967533 cites W2001583897 @default.
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- W3036967533 doi "https://doi.org/10.1017/s0424820100162351" @default.
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