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- W3037025699 abstract "The objective of this study was to investigate the counteracting effects of glycerol or rumen-protected choline chloride supplementation on rumen fermentation and the profile changes in blood metabolites in pregnant ewes suffering from negative energy balance. Eighteen pregnant Hu sheep with a gestation period of 100 days were used in the present study. Feed restriction was started from 100 days of gestation and lasted for 9 days. During the period of feed restriction, these sheep were randomly assigned to three groups: the control group (CON, n = 6), which received no supplementation, the glycerol group (GLY, n = 6), which received 40 mL of glycerol per day, and the rumen-protected choline chloride group (RPC, n = 6), which received 10 g of rumen-protected choline chloride per day lasted 9 days. All supplements were mixed with feed. The results showed that, compared to CON, glycerol supplementation decreased the proportion of acetate (P < 0.01) and increased the proportion of propionate (P < 0.01). The proportions of butyrate (P = 0.04) and valerate (P = 0.04) were higher in GLY than in RPC, but there was no significant difference in their proportions between GLY and CON. Blood β-hydroxybutyric acid concentration continuously increased in CON during the treatment period, while it remained stable in the ewes receiving glycerol or rumen-protected choline chloride. Glucose levels in the blood of ewes receiving glycerol or rumen-protected choline chloride were higher than those in CON (P = 0.01). Compared to CON, the levels of non-esterified fatty acids (NEFA) (P = 0.02) and very-low-density lipoproteins (VLDL) (P = 0.04) in the blood were increased in ewes that received rumen-protected choline chloride. Correlation analysis of the DEMs and rumen fermentation parameters showed a positive correlation between citric acid and phosphohydroxypyruvic acid (r = 0.61, P < 0.01), as well as between bilirubin and BHBA (r = 0.59, P < 0.01). d-Glucose have a positive correlation with propionate (r = 0.48, P = 0.02), while have a negative correlation with BHBA (r = −0.77, P < 0.01), 2-hydroxylauroylcarnitine (r = -0.53, P < 0.01), 2-octenoylcarnitine (r = −0.61, P < 0.01), and 3-hydroxydodecanoic acid (r = −0.60, P = 0.04). It is concluded that the supplementation of glycerol or rumen-protected choline chloride counteracted changes in metabolite concentrations in ewes that had been induced by feed restriction." @default.
- W3037025699 created "2020-07-02" @default.
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- W3037025699 date "2020-10-01" @default.
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- W3037025699 title "The effect of glycerol or rumen-protected choline chloride on rumen fermentation and blood metabolome in pregnant ewes suffering from negative energy balance" @default.
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- W3037025699 doi "https://doi.org/10.1016/j.anifeedsci.2020.114594" @default.
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