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• W3037039906 endingPage "100130" @default.
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• W3037039906 abstract "The current study aimed to evaluate the efficiency of dietary nucleotides-supplementation on broiler chickens to alleviate the intestinal Clostridium perfringens (C. perfringens) levels and its adverse effect on gut and growth performance parameters. In this study, a total of 270 one-day-old mixed broiler chicks (Cobb 500) were randomly divided into six treatment groups with three replicates of 15 chicks/ replicate. Treatment 1 (CX), a negative control group was fed corn-soybean basal diet without added nucleotides. Treatment 2 (CN 0.05) and treatment 3 (CN 0.1), consisted of chicks were fed the basal diet with the addition of nucleotides on top at two levels (0.05 and 0.1%) respectively. Treatment 4 (PX), treatment 5 (PN 0.05), and treatment 6 (PN 0.1) consisted of chicks that were challenged with C. perfringens inoculum (~4 × 108 CFU/ml) on day 14, 15, 16 and 17of the experiment and were fed diets similar to treatments 1, 2, and 3 respectively. The trial continued for 35 days. At the end of the experiment, the intestinal C. perfringens counts, microscopic lesion scores, intestinal histomorphology, intestinal barriers (occludin and mucin mRNA expression) and growth parameters were determined. The results showed that the pathogen challenge significantly (P˂0.05) increased both C. perfringens levels and intestinal lesion scores. Which adversely affects intestinal barriers and intestinal histomorphology resulting in a significant decrease (P˂0.05) in body weight gain (BWG) with an increase in feed conversion ratio (FCR). Whereas, nucleotides-supplementation, at 0.1%, significantly decreased both C. perfringens levels and intestinal lesion scores, and significantly improved intestinal barriers and intestinal histomorphology which consequently resulted in improved growth performance parameters to be nearly the same as that of the control un-supplemented group. In conclusion, nucleotides markedly ameliorated the negative effects of C. perfringens challenge by improving the intestinal barrier function and intestinal histomorphology which positively reflected on the growth performance of challenged birds." @default.
• W3037039906 created "2020-07-02" @default.
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• W3037039906 date "2020-12-01" @default.
• W3037039906 modified "2023-10-17" @default.
• W3037039906 title "The impact of exogenous dietary nucleotides in ameliorating Clostridium perfringens infection and improving intestinal barriers gene expression in broiler chicken" @default.
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• W3037039906 doi "https://doi.org/10.1016/j.vas.2020.100130" @default.
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