FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037108117> ?p ?o ?g. }

• W3037108117 endingPage "35" @default.
• W3037108117 startingPage "35" @default.
• W3037108117 abstract "Purpose: To investigate whether plasma rich in growth factors (PRGF) eye drops maintain their biological potential after a freeze drying process. The addition of a lyoprotectant like trehalose was also evaluated. Methods: Blood from three healthy donors was collected to obtain eye drops by PRGF technology. The resultant eye drops were divided in four groups: PRGF, freeze-dried PRGF (PRGF lyo), and PRGF lyophilized mixed with 2,5% trehalose (PRGF lyo+2.5T) or 5% trehalose (PRGF lyof+5T). Chemical and biological characteristics were evaluated. Photorefractive keratectomy was performed on C57BL/6 mice which were divided in three treatment groups: control, PRGF, and PRGF lyo. Corneal wound healing and haze formation were evaluated macroscopically. Eyes were collected at 1, 2, 3, and 7 days after surgery, and were processed for histologic studies. Results: The pH values of PRGF samples increased significantly after the lyophilization process. Osmolarity levels increased significantly in PRGF samples mixed with trehalose in comparison with PRGF samples without protectants. The freeze drying process maintained growth factors levels as well as the biological properties of PRGF eye drops even without the use of lyoprotectants. PRGF lyo treatment significantly decreased the re-epithelialization time and haze formation in photorefractive keratectomy-treated corneas regarding PRGF and control groups. Furthermore, the PRGF lyo group significantly decreased the number of smooth muscle actin-positive cells in comparison with the control group at each time of the study and at days 2 and 3 in the PRGF group. Conclusions: The freeze drying process preserves the protein and growth factor content as well as the biological properties of PRGF eye drops, even without the use of protectants. Freeze-dried PRGF eye drops accelerate corneal tissue regeneration after photorefractive keratectomy in comparison with the control group. Translational Relevance: Our study shows the feasibility to preserve the biological capability of PRGF eye drops as freeze-dried formulation, avoiding the addition of protectants." @default.
• W3037108117 created "2020-07-02" @default.
• W3037108117 creator A5003483976 @default.
• W3037108117 creator A5036174505 @default.
• W3037108117 creator A5052598654 @default.
• W3037108117 creator A5054189977 @default.
• W3037108117 creator A5062744013 @default.
• W3037108117 creator A5080129979 @default.
• W3037108117 date "2020-06-25" @default.
• W3037108117 modified "2023-10-18" @default.
• W3037108117 title "Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology" @default.
• W3037108117 cites W1967791041 @default.
• W3037108117 cites W1969393655 @default.
• W3037108117 cites W1973633538 @default.
• W3037108117 cites W1974441525 @default.
• W3037108117 cites W1982767852 @default.
• W3037108117 cites W1998361394 @default.
• W3037108117 cites W2005135342 @default.
• W3037108117 cites W2005722136 @default.
• W3037108117 cites W2021034206 @default.
• W3037108117 cites W2021037093 @default.
• W3037108117 cites W2021968547 @default.
• W3037108117 cites W2029515932 @default.
• W3037108117 cites W2068059127 @default.
• W3037108117 cites W2069786094 @default.
• W3037108117 cites W2081033328 @default.
• W3037108117 cites W2124985122 @default.
• W3037108117 cites W2143020633 @default.
• W3037108117 cites W2160770502 @default.
• W3037108117 cites W2163780750 @default.
• W3037108117 cites W2200925402 @default.
• W3037108117 cites W2263803520 @default.
• W3037108117 cites W2280312247 @default.
• W3037108117 cites W2321285499 @default.
• W3037108117 cites W2330742974 @default.
• W3037108117 cites W2407522371 @default.
• W3037108117 cites W2424789217 @default.
• W3037108117 cites W2471361232 @default.
• W3037108117 cites W260304762 @default.
• W3037108117 cites W2607686665 @default.
• W3037108117 cites W2625015844 @default.
• W3037108117 cites W2771070472 @default.
• W3037108117 cites W2794261825 @default.
• W3037108117 cites W2801711496 @default.
• W3037108117 cites W2807590117 @default.
• W3037108117 cites W3011455960 @default.
• W3037108117 cites W4205594235 @default.
• W3037108117 cites W4213281088 @default.
• W3037108117 cites W4231061328 @default.
• W3037108117 cites W4235124473 @default.
• W3037108117 cites W4235829314 @default.
• W3037108117 cites W4239796515 @default.
• W3037108117 cites W4242236000 @default.
• W3037108117 cites W4249452340 @default.
• W3037108117 cites W4255365342 @default.
• W3037108117 doi "https://doi.org/10.1167/tvst.9.7.35" @default.
• W3037108117 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7414653" @default.
• W3037108117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32832240" @default.
• W3037108117 hasPublicationYear "2020" @default.
• W3037108117 type Work @default.
• W3037108117 sameAs 3037108117 @default.
• W3037108117 citedByCount "8" @default.
• W3037108117 countsByYear W30371081172021 @default.
• W3037108117 countsByYear W30371081172022 @default.
• W3037108117 countsByYear W30371081172023 @default.
• W3037108117 crossrefType "journal-article" @default.
• W3037108117 hasAuthorship W3037108117A5003483976 @default.
• W3037108117 hasAuthorship W3037108117A5036174505 @default.
• W3037108117 hasAuthorship W3037108117A5052598654 @default.
• W3037108117 hasAuthorship W3037108117A5054189977 @default.
• W3037108117 hasAuthorship W3037108117A5062744013 @default.
• W3037108117 hasAuthorship W3037108117A5080129979 @default.
• W3037108117 hasBestOaLocation W30371081171 @default.
• W3037108117 hasConcept C118487528 @default.
• W3037108117 hasConcept C185592680 @default.
• W3037108117 hasConcept C192562407 @default.
• W3037108117 hasConcept C2776882836 @default.
• W3037108117 hasConcept C2778452849 @default.
• W3037108117 hasConcept C2780603455 @default.
• W3037108117 hasConcept C43617362 @default.
• W3037108117 hasConcept C55493867 @default.
• W3037108117 hasConcept C71924100 @default.
• W3037108117 hasConcept C84199377 @default.
• W3037108117 hasConceptScore W3037108117C118487528 @default.
• W3037108117 hasConceptScore W3037108117C185592680 @default.
• W3037108117 hasConceptScore W3037108117C192562407 @default.
• W3037108117 hasConceptScore W3037108117C2776882836 @default.
• W3037108117 hasConceptScore W3037108117C2778452849 @default.
• W3037108117 hasConceptScore W3037108117C2780603455 @default.
• W3037108117 hasConceptScore W3037108117C43617362 @default.
• W3037108117 hasConceptScore W3037108117C55493867 @default.
• W3037108117 hasConceptScore W3037108117C71924100 @default.
• W3037108117 hasConceptScore W3037108117C84199377 @default.
• W3037108117 hasIssue "7" @default.
• W3037108117 hasLocation W30371081171 @default.
• W3037108117 hasLocation W30371081172 @default.
• W3037108117 hasLocation W30371081173 @default.
• W3037108117 hasOpenAccess W3037108117 @default.

• next