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- W3037138813 abstract "Abstract The breast cancer susceptibility protein 1 (BRCA1) plays a central role in the suppression of human breast and ovarian cancer. Germ line mutations of the BRCA1 gene are responsible for the hereditary breast and ovarian cancer (HBOC) syndrome. Here were report 1 H, 13 C, and 15 N resonance assignments for the intrinsically disordered BRCA1 fragment 219–504, which contains important interaction sites for the proto-oncogenic transcription factor MYC as well as for p53. A nuclear magnetic resonance assignment was achieved at 18.8 T magnetic field strength using a 5D HN(CA)CONH experiment and its associated 4D H(NCA)CONH and 4D (H)N(CA)CONH experiments. 13 C α and 13 C β assignments were obtained using a 5D HabCabCONH experiment. With this strategy, 90% of 1 H/ 15 N backbone pairs could be assigned. Similarly, 264 C’ resonances were assigned corresponding to 86% of the total number of C’ atoms. In addition, 252 C β resonances (i.e. 85%) were assigned, together with 461 attached H β nuclei, as well as 264 (i.e. 86%) C α resonances, together with 275 attached H α nuclei." @default.
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- W3037138813 date "2020-06-24" @default.
- W3037138813 modified "2023-10-08" @default.
- W3037138813 title "1H, 13C and 15N backbone resonance assignment of BRCA1 fragment 219–504" @default.
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- W3037138813 doi "https://doi.org/10.1007/s12104-020-09963-6" @default.
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