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• W3037287718 endingPage "1684" @default.
• W3037287718 startingPage "1684" @default.
• W3037287718 abstract "The immunophenotype is a key element to classify B-cell Non-Hodgkin Lymphomas (B-NHL); while it is routinely obtained through immunohistochemistry, the use of flow cytometry (FC) could bear several advantages. However, few FC laboratories can rely on a long-standing practical experience, and the literature in support is still limited; as a result, the use of FC is generally restricted to the analysis of lymphomas with bone marrow or peripheral blood involvement. In this work, we applied machine learning to our database of 1465 B-NHL samples from different sources, building four artificial predictive systems which could classify B-NHL in up to nine of the most common clinico-pathological entities. Our best model shows an overall accuracy of 92.68%, a mean sensitivity of 88.54% and a mean specificity of 98.77%. Beyond the clinical applicability, our models demonstrate (i) the strong discriminatory power of MIB1 and Bcl2, whose integration in the predictive model significantly increased the performance of the algorithm; (ii) the potential usefulness of some non-canonical markers in categorizing B-NHL; and (iii) that FC markers should not be described as strictly positive or negative according to fixed thresholds, but they rather correlate with different B-NHL depending on their level of expression." @default.
• W3037287718 created "2020-07-02" @default.
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• W3037287718 date "2020-06-24" @default.
• W3037287718 modified "2023-10-05" @default.
• W3037287718 title "A Clinically Applicable Approach to the Classification of B-Cell Non-Hodgkin Lymphomas with Flow Cytometry and Machine Learning" @default.
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• W3037287718 doi "https://doi.org/10.3390/cancers12061684" @default.
• W3037287718 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7352227" @default.
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• W3037287718 hasPublicationYear "2020" @default.
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