Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037290400> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W3037290400 endingPage "321" @default.
- W3037290400 startingPage "313" @default.
- W3037290400 abstract "The ITS-DNA region of Synchytrium endobioticum (Schilb.) Pere., rhe causal agent of poraro wart disease, was used ro generate specific PCR primers for molecular diagnosis of rhe disease. DNA was extracted from wart galls of all S. endobioticum pathotypes (I, 2, 6, 18) currently occurring and used for official resting purposes in Germany. Using the universal ITS primer # 4 and the S. endobioticumspecific primer Kbrl, a PCR fragment of 543 bp was obtained from all four fungal pathocypes. DNA for PCR-based diagnosis of the fungus could be extracted easily from summer sori, however, we did nor succeed ro extract amplifiable DNA from resting sori found in contaminated soils. To circumvent rhis problem, zoospores emerging from resring sori were used for DNA exrracrion and indirect detection. PCR also allowed ro discriminate between weakly resistant and moderately susceptible responses of potaro culrivars in addition ro rhe routine visual inspection, since only alive summer sori of the weakly susceptible reacting poraro culrivars released quantities of zoospores sufficient ro be detected. No PCR signal was obtained from weakly or completely resistant potaro cultivars." @default.
- W3037290400 created "2020-07-02" @default.
- W3037290400 creator A5005477876 @default.
- W3037290400 creator A5084061479 @default.
- W3037290400 date "2004-01-01" @default.
- W3037290400 modified "2023-09-24" @default.
- W3037290400 title "PCR-detection of Synchytrium endobioticum (Schilb.) Pere." @default.
- W3037290400 hasPublicationYear "2004" @default.
- W3037290400 type Work @default.
- W3037290400 sameAs 3037290400 @default.
- W3037290400 citedByCount "0" @default.
- W3037290400 crossrefType "journal-article" @default.
- W3037290400 hasAuthorship W3037290400A5005477876 @default.
- W3037290400 hasAuthorship W3037290400A5084061479 @default.
- W3037290400 hasConcept C113735248 @default.
- W3037290400 hasConcept C153911025 @default.
- W3037290400 hasConcept C174618031 @default.
- W3037290400 hasConcept C178790620 @default.
- W3037290400 hasConcept C185592680 @default.
- W3037290400 hasConcept C2777563447 @default.
- W3037290400 hasConcept C54355233 @default.
- W3037290400 hasConcept C552990157 @default.
- W3037290400 hasConcept C59822182 @default.
- W3037290400 hasConcept C86803240 @default.
- W3037290400 hasConceptScore W3037290400C113735248 @default.
- W3037290400 hasConceptScore W3037290400C153911025 @default.
- W3037290400 hasConceptScore W3037290400C174618031 @default.
- W3037290400 hasConceptScore W3037290400C178790620 @default.
- W3037290400 hasConceptScore W3037290400C185592680 @default.
- W3037290400 hasConceptScore W3037290400C2777563447 @default.
- W3037290400 hasConceptScore W3037290400C54355233 @default.
- W3037290400 hasConceptScore W3037290400C552990157 @default.
- W3037290400 hasConceptScore W3037290400C59822182 @default.
- W3037290400 hasConceptScore W3037290400C86803240 @default.
- W3037290400 hasIssue "4" @default.
- W3037290400 hasLocation W30372904001 @default.
- W3037290400 hasOpenAccess W3037290400 @default.
- W3037290400 hasPrimaryLocation W30372904001 @default.
- W3037290400 hasRelatedWork W1475642743 @default.
- W3037290400 hasRelatedWork W1490114307 @default.
- W3037290400 hasRelatedWork W1971786454 @default.
- W3037290400 hasRelatedWork W2052784048 @default.
- W3037290400 hasRelatedWork W2060571288 @default.
- W3037290400 hasRelatedWork W2063417130 @default.
- W3037290400 hasRelatedWork W2073250183 @default.
- W3037290400 hasRelatedWork W2085590020 @default.
- W3037290400 hasRelatedWork W2098865256 @default.
- W3037290400 hasRelatedWork W2105860904 @default.
- W3037290400 hasRelatedWork W2106808433 @default.
- W3037290400 hasRelatedWork W2126595119 @default.
- W3037290400 hasRelatedWork W2208525400 @default.
- W3037290400 hasRelatedWork W2377507320 @default.
- W3037290400 hasRelatedWork W2472818867 @default.
- W3037290400 hasRelatedWork W261628968 @default.
- W3037290400 hasRelatedWork W2468612036 @default.
- W3037290400 hasRelatedWork W3144154706 @default.
- W3037290400 hasVolume "111" @default.
- W3037290400 isParatext "false" @default.
- W3037290400 isRetracted "false" @default.
- W3037290400 magId "3037290400" @default.
- W3037290400 workType "article" @default.