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- W3037301628 abstract "Identifying anddetecting health hazards in food products, especially contaminants and toxicsubstances such as allergens, food toxins and agricultural residues frompesticides, remains a challenge. Increasing demand for food products andgrowing health consciousness necessitate rapid and accuratemeasurements which can be easily conducted on-site without long measurementtimes and high costs. Due to their ease of use, accuracy sample preparation andrapidity, biosensors have started to outcompete time-consuming lab-scale analyticaldevices. However, as the use of biosensors increase, a concern of theamount of plastics and synthetic polymers used in the fabrication of thesebiosensors rises. In this dissertation, new ways to create biodegradable andeco-friendly plant-based SERS biosensor platforms from corn protein, zein, arepresented. Its higher hydrophobicity and film forming capability make zein avery suitable biopolymer for fabricating biosensors. In the first part of thisdissertation, chemical crosslinking was tested to improve the surfacehydrophobicity, surface roughness (using AFM), mechanical properties, kineticsof gelation and film formation of zein films, and as a result zein-film basedSERS platforms with fewer defects couldbe fabricated. In the second part, the detection sensitivity of the zein film-basedSERS platforms was increased with metallic nanoparticle decoration (gold, silveror silver-shelled-gold). The addition of all three types of nanoparticlessignificantly increased the SERS enhancement factors of the platforms, withsilver-shelled-gold nanoparticles giving the highest enhancement factor of 105.In the last part of this thesis, a novel approach was tested, where electrospun zeinnanofibers decorated with metallic nanoparticles were used as a SERS biosensorplatform. Due to their higher surface area-to-volume ratios, electrospun zeinnanofibers gave a higher SERS enhancement factor (106). Thisenhancement factor enabled the detection of acrylamide, a food carcinogen, witha 104 times lower detection limit than nanophotonicbased nanoimprinted zein, acrylamide sensor platform. Overall,this dissertation successfully shows the fabrication of biodegradable andeco-friendly SERS sensor platforms that have comparable detection sensitivitiesto those of non-biodegradable ones." @default.
- W3037301628 created "2020-07-02" @default.
- W3037301628 creator A5021194943 @default.
- W3037301628 date "2020-06-26" @default.
- W3037301628 modified "2023-09-23" @default.
- W3037301628 title "Fabrication of zein-based biodegradable surface enhanced Raman spectroscopy biosensor platforms for the detection of food toxins" @default.
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- W3037301628 doi "https://doi.org/10.25394/pgs.12567728.v1" @default.
- W3037301628 hasPublicationYear "2020" @default.
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