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• W3037331781 endingPage "4721" @default.
• W3037331781 startingPage "4705" @default.
• W3037331781 abstract "HIV-1 entry into cells is mediated by the fusion protein gp41. Cholesterol plays an important role in this virus-cell fusion, but molecular structural information about cholesterol-gp41 interaction is so far absent. Here, we present experimental and computational data about cholesterol complexation with gp41 in lipid bilayers. We focus on the C-terminal region of the protein, which comprises a membrane-proximal external region (MPER) and the transmembrane domain (TMD). We measured peptide-cholesterol contacts in virus-mimetic lipid bilayers using solid-state NMR spectroscopy, and augmented these experimental data with all-atom molecular dynamics simulations. 2D 19F NMR spectra show correlation peaks between MPER residues and the cholesterol isooctyl tail, indicating that cholesterol is in molecular contact with the MPER-TMD trimer. 19F-13C distance measurements between the peptide and 13C-labeled cholesterol show that C17 on the D ring and C9 at the intersection of B and C rings are ~7.0 A from the F673 side-chain 4-19F. At high peptide concentrations in the membrane, the 19F-13C distance data indicate three cholesterol molecules bound near F673 in each trimer. Mutation of a cholesterol recognition amino acid consensus motif did not change these distances, indicating that cholesterol binding does not require this sequence motif. Molecular dynamics simulations further identify two hotspots for cholesterol interactions. Taken together, these experimental data and simulations indicate that the helix-turn-helix conformation of the MPER-TMD is responsible for sequestering cholesterol. We propose that this gp41-cholesterol interaction mediates virus-cell fusion by recruiting gp41 to the boundary of the liquid-disordered and liquid-ordered phases to incur membrane curvature." @default.
• W3037331781 created "2020-07-02" @default.
• W3037331781 creator A5007765901 @default.
• W3037331781 creator A5013024407 @default.
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• W3037331781 creator A5083231687 @default.
• W3037331781 creator A5084956627 @default.
• W3037331781 date "2020-07-01" @default.
• W3037331781 modified "2023-10-13" @default.
• W3037331781 title "Cholesterol Interaction with the Trimeric HIV Fusion Protein gp41 in Lipid Bilayers Investigated by Solid-State NMR Spectroscopy and Molecular Dynamics Simulations" @default.
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• W3037331781 doi "https://doi.org/10.1016/j.jmb.2020.06.017" @default.
• W3037331781 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7781112" @default.
• W3037331781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32592698" @default.
• W3037331781 hasPublicationYear "2020" @default.
• W3037331781 type Work @default.
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