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• W3037442666 abstract "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily affects the respiratory system, although the involvement of other organs, such as heart, kidney, and bowel, has also been observed.1Chen N Zhou M Dong X et al.Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study.Lancet. 2020; 395: 507‐513Abstract Full Text Full Text PDF PubMed Scopus (13343) Google Scholar, 2Singhal T A review of coronavirus disease-2019 (COVID-19).Indian J Pediatr. 2020; 87: 281‐286Crossref PubMed Scopus (2013) Google Scholar, 3Yuki K Fujiogi M Koutsogiannaki S COVID-19 pathophysiology: a review.Clin Immunol. 2020; 215108427Crossref PubMed Scopus (1104) Google Scholar, 4Huang C Wang Y Li X et al.Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.Lancet. 2020; 395: 497‐506Abstract Full Text Full Text PDF PubMed Scopus (30327) Google Scholar, 5Jiang F Deng L Zhang L Cai Y Cheung CW Xia Z Review of the clinical characteristics of coronavirus disease 2019 (COVID-19).J Gen Intern Med. 2020; 35: 1545‐1549Crossref PubMed Scopus (852) Google Scholar We conducted a retrospective study on 61 patients with coronavirus disease 19 (COVID-19) admitted to Desio Hospital, Lombardy, Italy, from February 1, 2020 to April 30, 2020 to assess bacterial and fungal pulmonary colonization. In accordance with WHO guidance, only SARS-CoV-2 cases confirmed through real-time reverse-transcriptase–polymerase-chain-reaction assays on nasopharyngeal swabs were included in the analysis.6WHOLaboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases.Interim Guid. 2020; 5: 1-7Google Scholar All patients were admitted to the intensive care unit (ICU) due to the presence of acute hypoxemic respiratory failure that required invasive respiratory supports, such as mechanical ventilation and high level of positive end-expiratory pressure. Here, we investigated the relationship between SARS-CoV-2 and bacterial and fungal colonization using data of first bronchial aspirate cultures of each patient, 35 (57%) of which resulted positive for bacterial or fungal infection. The species identification was performed by Vitek Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry system (bioMérieux). Pathogenic fungi were isolated in 19 specimens: 14 Candida albicans (40%), 4 Candida glabrata (11.4%), and 1 Aspergillus fumigatus (3%). In other 13 samples, we identified Pseudomonas aeruginosa (n = 6, 17%), Staphylococcus aureus (n = 2, 5%), Klebsiella pneumoniae (n = 1, 3%), Escherichia coli (n = 1, 3%), Klebsiella oxytoca (n = 1, 3%), Enterobacter cloacae (n = 1, 3%), or Staphylococcus epidermidis (n = 1, 3%). Finally, in 3 (8.6%) samples, we identified both C. albicans and P. aeruginosa. Antimicrobial susceptibility and resistance detection of the clinical isolates were determined using Vitek cards (bioMérieux, Marcy l'Etoile, France). No multidrug- resistant bacteria or fungi were isolated. Reviewing the antimicrobial resistance data of bronchial aspirate isolates of patients hospitalized in the ICU in 2019, we found that 17% of the isolated strains was resistant to beta-lactam drugs, vancomycin, carbapenem drugs, or methicillin. This observation is in contrast with the results observed for the strains isolated from severe COVID-19 patients, suggesting that the bacterial and fungal colonization in these subjects was not of nosocomial origin. In this study, among the 35 patients presenting lung SARS-CoV-2 infection and concomitant positive bronchial aspirates, 28 (80%) were colonized by either fungi or P. aeruginosa. Fungi are the major causes of morbidity and mortality in immunocompromised subjects, while P. aeruginosa is the most common gram-negative pathogen causing pneumonia associated with worse clinical outcomes.7Jiang S Immunity against Fungal Infections.Immunol Immunogenet Insights. 2016; 8: 3-6Crossref Scopus (6) Google Scholar,8Curran CS Bolig T Torabi-Parizi P Mechanisms and targeted therapies for Pseudomonas aeruginosa lung infection.Am J Respir Crit Care Med. 2018; 197: 708‐727Crossref PubMed Scopus (83) Google Scholar On the other hand, in non-COVID-19 patients admitted to the ICU in 2019, only 20% of first bronchial aspirate cultures presented fungi (C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, and A. fumigatus) or P. aeruginosa colonization. SARS-CoV-2 enters the target cells via the angiotensin-converting enzyme 2 receptor, which is highly expressed on alveolar epithelial cells, but also on heart, kidney, and intestinal cells.3Yuki K Fujiogi M Koutsogiannaki S COVID-19 pathophysiology: a review.Clin Immunol. 2020; 215108427Crossref PubMed Scopus (1104) Google Scholar,9Sarzi-Puttini P Giorgi V Sirotti S et al.COVID-19, cytokines and immunosuppression: what can we learn from severe acute respiratory syndrome?.Clin Exp Rheumatol. 2020; 38: 337-342Crossref PubMed Google Scholar A constellation of innate immune cells (neutrophils and monocytes) and adaptive immune cells (particularly CD4+ T cells and CD8+ T cells) are involved in the response to viral infections, and COVID-19 is no exception. It has been reported that SARS-CoV-2 strongly activates the immune system inducing an abnormal cytokine production known as “cytokine storm,” especially in severe cases.10Mehta P McAuley DF Brown M et al.COVID-19: consider cytokine storm syndromes and immunosuppression.Lancet. 2020; 395: 1033‐1034Abstract Full Text Full Text PDF PubMed Scopus (6293) Google Scholar Indeed, subjects with severe COVID-19 exhibited increased plasma levels of pro-inflammatory cytokines and chemokines, and reduced T-cells number in peripheral blood, potentially as consequence of lymphocyte accumulation to the site of infection.3Yuki K Fujiogi M Koutsogiannaki S COVID-19 pathophysiology: a review.Clin Immunol. 2020; 215108427Crossref PubMed Scopus (1104) Google Scholar,11Li G Fan Y Lai Y et al.Coronavirus infections and immune responses.J Med Virol. 2020; 92: 424-432Crossref PubMed Scopus (1201) Google Scholar Such elevated immune response can kill infected cells but can also contribute to the aggravation of the disease and to lung injury.3Yuki K Fujiogi M Koutsogiannaki S COVID-19 pathophysiology: a review.Clin Immunol. 2020; 215108427Crossref PubMed Scopus (1104) Google Scholar,11Li G Fan Y Lai Y et al.Coronavirus infections and immune responses.J Med Virol. 2020; 92: 424-432Crossref PubMed Scopus (1201) Google Scholar It is worth noticing that, in severe COVID-19 patients, beside respiratory symptoms, thrombosis and pulmonary embolism have been observed.3Yuki K Fujiogi M Koutsogiannaki S COVID-19 pathophysiology: a review.Clin Immunol. 2020; 215108427Crossref PubMed Scopus (1104) Google Scholar More in-depth studies are needed to identify the molecular players involved in SARS-CoV-2 pathogenesis, which might reveal key targets to reduce or inhibit the cytokine storm. In healthy individuals, Candida species and P. aeruginosa can colonize mucous membranes and skin, and both innate and adaptive immune cells contribute to the antifungal and antibacterial defense.7Jiang S Immunity against Fungal Infections.Immunol Immunogenet Insights. 2016; 8: 3-6Crossref Scopus (6) Google Scholar,8Curran CS Bolig T Torabi-Parizi P Mechanisms and targeted therapies for Pseudomonas aeruginosa lung infection.Am J Respir Crit Care Med. 2018; 197: 708‐727Crossref PubMed Scopus (83) Google Scholar,12Netea MG Joosten LA van der Meer JW Kullberg BJ van de Veerdonk FL Immune defense against Candida fungal infections.Nat Rev Immunol. 2015; 15: 630‐642Crossref Scopus (349) Google Scholar Neutrophils, macrophages, dendritic cells, and T- and B-lymphocytes are the major cellular players. The innate immune cells are the first line of defense, and the release of inflammatory cytokines and chemokines induces the recruitment of neutrophils from the peripheral blood. Dendritic cells are particularly important to initiate the adaptive immune response. Among adaptive immune cells, CD4+ T helper cells play a key role with Th17 being the most relevant subtype. These cells act principally at the lung mucosal barrier, and produce/release interleukin-17 (IL-17), which contributes to the enhanced organization of B- and T-cells into bronchus-associated lymphoid tissue involved in mediating secondary immune responses, and the release of antifungal β-defensins. Indeed, patients with deficiency in IL-17 production or signaling appear to be more susceptible to mucosal fungal and P. aeruginosa infections, as previously reported.7Jiang S Immunity against Fungal Infections.Immunol Immunogenet Insights. 2016; 8: 3-6Crossref Scopus (6) Google Scholar,8Curran CS Bolig T Torabi-Parizi P Mechanisms and targeted therapies for Pseudomonas aeruginosa lung infection.Am J Respir Crit Care Med. 2018; 197: 708‐727Crossref PubMed Scopus (83) Google Scholar,12Netea MG Joosten LA van der Meer JW Kullberg BJ van de Veerdonk FL Immune defense against Candida fungal infections.Nat Rev Immunol. 2015; 15: 630‐642Crossref Scopus (349) Google Scholar In our hospital, we observed an increased prevalence of fungal and P. aeruginosa colonization in severe COVID-19 patients compared to non-COVID-19 cases. It could be speculated that this association might be the result of the over-activation of the immune system causing the failure in the regulation of the defenses against pathogens other than SARS-CoV-2, and the progression to co-infections and therefore to lung injury. Certainly, multicenter studies with a larger number of subjects are needed to verify and improve our results. However, this study highlights the importance of not to neglect fungal and bacterial lung colonization in severe COVID-19 cases that, along with the detection of molecules involved in the immune response and in the mechanisms of the host-pathogen interaction, can be useful for the development of personalized therapies and to improve patients’ management in the ICU. We gratefully acknowledge Dr Elena Intra for reviewing the manuscript." @default.
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• W3037442666 title "Bacterial and fungal colonization of the respiratory tract in COVID-19 patients should not be neglected" @default.
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