Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037722082> ?p ?o ?g. }
- W3037722082 abstract "Abstract The anteroposterior axial identity of motor neurons (MNs) determines their functionality and vulnerability to neurodegeneration. Thus it is a critical parameter in the design of strategies aiming to produce MNs from human pluripotent stem cells (hPSCs) for regenerative medicine and disease modelling applications. However, the in vitro generation of posterior spinal cord MNs has been challenging. Although the induction of cells resembling neuromesodermal progenitors (NMPs), the bona fide precursors of the mammalian spinal cord, offers a promising solution, the progressive specification of posterior MNs from these cells is not well-defined. Here we determine the signals guiding the transition of human NMP-like cells toward posterior ventral spinal cord neurectoderm. We show that combined WNT-FGF activities drive a posterior dorsal early neural state while suppression of TGFβ-BMP signalling pathways, combined with SHH stimulation, promotes a ventral identity. Based on these results, we define an optimised protocol for the generation of posterior MNs that can efficiently integrate within the neural tube of chick embryos. We expect that our findings will facilitate the functional comparison of hPSC-derived spinal cord cells of distinct axial identities." @default.
- W3037722082 created "2020-07-02" @default.
- W3037722082 creator A5023713760 @default.
- W3037722082 creator A5027202141 @default.
- W3037722082 creator A5035797757 @default.
- W3037722082 creator A5049984313 @default.
- W3037722082 creator A5050907953 @default.
- W3037722082 creator A5066046626 @default.
- W3037722082 creator A5069996996 @default.
- W3037722082 creator A5071054395 @default.
- W3037722082 creator A5076461646 @default.
- W3037722082 creator A5079234046 @default.
- W3037722082 creator A5083138771 @default.
- W3037722082 date "2020-06-24" @default.
- W3037722082 modified "2023-10-16" @default.
- W3037722082 title "Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives" @default.
- W3037722082 cites W110925524 @default.
- W3037722082 cites W1500747401 @default.
- W3037722082 cites W1537836582 @default.
- W3037722082 cites W1931118778 @default.
- W3037722082 cites W1968707675 @default.
- W3037722082 cites W1977350867 @default.
- W3037722082 cites W1977374662 @default.
- W3037722082 cites W1977735902 @default.
- W3037722082 cites W1977898964 @default.
- W3037722082 cites W1985023917 @default.
- W3037722082 cites W1987865998 @default.
- W3037722082 cites W1992681202 @default.
- W3037722082 cites W1993982470 @default.
- W3037722082 cites W1999574084 @default.
- W3037722082 cites W2000101032 @default.
- W3037722082 cites W2001296529 @default.
- W3037722082 cites W2006047257 @default.
- W3037722082 cites W2013822134 @default.
- W3037722082 cites W2015799439 @default.
- W3037722082 cites W2017845174 @default.
- W3037722082 cites W2018288956 @default.
- W3037722082 cites W2020458486 @default.
- W3037722082 cites W2021759395 @default.
- W3037722082 cites W2033904138 @default.
- W3037722082 cites W2034609545 @default.
- W3037722082 cites W2035510388 @default.
- W3037722082 cites W2035988432 @default.
- W3037722082 cites W2039170732 @default.
- W3037722082 cites W2039722938 @default.
- W3037722082 cites W2043807089 @default.
- W3037722082 cites W2044962596 @default.
- W3037722082 cites W2047717163 @default.
- W3037722082 cites W2048350099 @default.
- W3037722082 cites W2049254977 @default.
- W3037722082 cites W2049993468 @default.
- W3037722082 cites W2058419637 @default.
- W3037722082 cites W2060230550 @default.
- W3037722082 cites W2061144812 @default.
- W3037722082 cites W2064520670 @default.
- W3037722082 cites W2066729924 @default.
- W3037722082 cites W2068342119 @default.
- W3037722082 cites W2072808488 @default.
- W3037722082 cites W2075322700 @default.
- W3037722082 cites W2079833508 @default.
- W3037722082 cites W2079883339 @default.
- W3037722082 cites W2082807347 @default.
- W3037722082 cites W2086669296 @default.
- W3037722082 cites W2086943348 @default.
- W3037722082 cites W2087206546 @default.
- W3037722082 cites W2087786585 @default.
- W3037722082 cites W2100555636 @default.
- W3037722082 cites W2100855116 @default.
- W3037722082 cites W2103412450 @default.
- W3037722082 cites W2103661362 @default.
- W3037722082 cites W2104962692 @default.
- W3037722082 cites W2107554012 @default.
- W3037722082 cites W2119666376 @default.
- W3037722082 cites W2119816938 @default.
- W3037722082 cites W2122980330 @default.
- W3037722082 cites W2123166021 @default.
- W3037722082 cites W2124649657 @default.
- W3037722082 cites W2128393954 @default.
- W3037722082 cites W2128464557 @default.
- W3037722082 cites W2128665076 @default.
- W3037722082 cites W2133437679 @default.
- W3037722082 cites W2135107819 @default.
- W3037722082 cites W2135447402 @default.
- W3037722082 cites W2135845674 @default.
- W3037722082 cites W2140034535 @default.
- W3037722082 cites W2142423248 @default.
- W3037722082 cites W2142514820 @default.
- W3037722082 cites W2142951125 @default.
- W3037722082 cites W2144274759 @default.
- W3037722082 cites W2152239989 @default.
- W3037722082 cites W2152426454 @default.
- W3037722082 cites W2153941478 @default.
- W3037722082 cites W2156688270 @default.
- W3037722082 cites W2160249662 @default.
- W3037722082 cites W2161645967 @default.
- W3037722082 cites W2162623914 @default.
- W3037722082 cites W2169456326 @default.
- W3037722082 cites W2171109270 @default.
- W3037722082 cites W2171674474 @default.
- W3037722082 cites W2179438025 @default.