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- W3037782904 abstract "Compartmentalization of cellular signaling forms the molecular basis of cellular behavior. The primary cilium constitutes a subcellular compartment that orchestrates signal transduction independent from the cell body. Ciliary dysfunction causes severe diseases, termed ciliopathies. Analyzing ciliary signaling has been challenging due to the lack of tools to investigate ciliary signaling. Here, we describe a nanobody-based targeting approach for optogenetic tools in mammalian cells and in vivo in zebrafish to specifically analyze ciliary signaling and function. Thereby, we overcome the loss of protein function observed after fusion to ciliary targeting sequences. We functionally localized modifiers of cAMP signaling, the photo-activated adenylyl cyclase bPAC and the light-activated phosphodiesterase LAPD, and the cAMP biosensor mlCNBD-FRET to the cilium. Using this approach, we studied the contribution of spatial cAMP signaling in controlling cilia length. Combining optogenetics with nanobody-based targeting will pave the way to the molecular understanding of ciliary function in health and disease." @default.
- W3037782904 created "2020-07-02" @default.
- W3037782904 creator A5016536397 @default.
- W3037782904 creator A5028883291 @default.
- W3037782904 creator A5040901959 @default.
- W3037782904 creator A5046756787 @default.
- W3037782904 creator A5050083499 @default.
- W3037782904 creator A5053586448 @default.
- W3037782904 creator A5062519728 @default.
- W3037782904 creator A5075651234 @default.
- W3037782904 creator A5077869340 @default.
- W3037782904 creator A5088264372 @default.
- W3037782904 creator A5090109226 @default.
- W3037782904 date "2020-06-24" @default.
- W3037782904 modified "2023-10-14" @default.
- W3037782904 title "Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium" @default.
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