Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037844516> ?p ?o ?g. }
- W3037844516 endingPage "110415" @default.
- W3037844516 startingPage "110415" @default.
- W3037844516 abstract "Tight junctions play an important role in maintaining barrier integrity of intestinal epithelia. Activation of AMP-activated protein kinase (AMPK) promotes tight junction assembly in intestinal epithelial cells (IEC). Fructo-oligosaccharides (FOS), well-known prebiotics, have previously been shown to alleviate inflammation-associated intestinal epithelial disruption although the mechanisms were unclear. This study aimed to investigate any effect of FOS on AMPK activity and tight junction assembly under non-inflammatory and inflammatory conditions using T84 cells as an IEC model. As analyzed by western blot, FOS induced AMPK activation through a calcium sensing receptor (CaSR)-phospholipase C (PLC)- Ca2+/calmodulin-dependent protein kinase kinase-β (CaMKKβ) pathway. Calcium switch assays and immunofluorescence staining of zonula occludens-1 (ZO-1) revealed that FOS induced tight junction assembly via an CaMKKβ-AMPK-dependent mechanism in IEC. Interestingly, FOS reversed the suppressive effect of lipopolysaccharide (LPS) on AMPK activity and tight junction assembly via a CaMKKβ pathway. Taken together, these findings uncover a prebiotic-independent effect of FOS in promoting intestinal epithelial tight junction assembly through AMPK activation, which may have implications for the treatment of diseases whose pathogenesis involves impaired intestinal barrier function." @default.
- W3037844516 created "2020-07-02" @default.
- W3037844516 creator A5031802823 @default.
- W3037844516 creator A5041854550 @default.
- W3037844516 creator A5058283826 @default.
- W3037844516 creator A5059512780 @default.
- W3037844516 creator A5061679017 @default.
- W3037844516 creator A5062223587 @default.
- W3037844516 creator A5064175599 @default.
- W3037844516 creator A5083564868 @default.
- W3037844516 date "2020-09-01" @default.
- W3037844516 modified "2023-10-07" @default.
- W3037844516 title "A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway" @default.
- W3037844516 cites W1541824835 @default.
- W3037844516 cites W1965888624 @default.
- W3037844516 cites W1979455982 @default.
- W3037844516 cites W1979518977 @default.
- W3037844516 cites W1981488904 @default.
- W3037844516 cites W1984640787 @default.
- W3037844516 cites W2034103681 @default.
- W3037844516 cites W2043933283 @default.
- W3037844516 cites W2049713190 @default.
- W3037844516 cites W2065586949 @default.
- W3037844516 cites W2067045943 @default.
- W3037844516 cites W2072015936 @default.
- W3037844516 cites W2077869163 @default.
- W3037844516 cites W2079199391 @default.
- W3037844516 cites W2081128554 @default.
- W3037844516 cites W2099540110 @default.
- W3037844516 cites W2105811469 @default.
- W3037844516 cites W2120479901 @default.
- W3037844516 cites W2121888268 @default.
- W3037844516 cites W2122112029 @default.
- W3037844516 cites W2140406095 @default.
- W3037844516 cites W2140460850 @default.
- W3037844516 cites W2155770880 @default.
- W3037844516 cites W2159992457 @default.
- W3037844516 cites W2168997831 @default.
- W3037844516 cites W2178956518 @default.
- W3037844516 cites W2211097356 @default.
- W3037844516 cites W2217281605 @default.
- W3037844516 cites W2294268013 @default.
- W3037844516 cites W2299295930 @default.
- W3037844516 cites W2531755827 @default.
- W3037844516 cites W2535932582 @default.
- W3037844516 cites W2548257578 @default.
- W3037844516 cites W2574756353 @default.
- W3037844516 cites W2585111741 @default.
- W3037844516 cites W2588900386 @default.
- W3037844516 cites W2594724854 @default.
- W3037844516 cites W2604209550 @default.
- W3037844516 cites W2764046617 @default.
- W3037844516 cites W2767047143 @default.
- W3037844516 cites W2789348215 @default.
- W3037844516 cites W2792859581 @default.
- W3037844516 cites W2793230973 @default.
- W3037844516 cites W2808183577 @default.
- W3037844516 cites W2838836163 @default.
- W3037844516 cites W2883222066 @default.
- W3037844516 cites W2884443157 @default.
- W3037844516 cites W2885971199 @default.
- W3037844516 cites W2900408542 @default.
- W3037844516 cites W2900594668 @default.
- W3037844516 cites W2968297580 @default.
- W3037844516 cites W3011820209 @default.
- W3037844516 cites W4293247451 @default.
- W3037844516 cites W866767112 @default.
- W3037844516 doi "https://doi.org/10.1016/j.biopha.2020.110415" @default.
- W3037844516 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32603892" @default.
- W3037844516 hasPublicationYear "2020" @default.
- W3037844516 type Work @default.
- W3037844516 sameAs 3037844516 @default.
- W3037844516 citedByCount "28" @default.
- W3037844516 countsByYear W30378445162020 @default.
- W3037844516 countsByYear W30378445162021 @default.
- W3037844516 countsByYear W30378445162022 @default.
- W3037844516 countsByYear W30378445162023 @default.
- W3037844516 crossrefType "journal-article" @default.
- W3037844516 hasAuthorship W3037844516A5031802823 @default.
- W3037844516 hasAuthorship W3037844516A5041854550 @default.
- W3037844516 hasAuthorship W3037844516A5058283826 @default.
- W3037844516 hasAuthorship W3037844516A5059512780 @default.
- W3037844516 hasAuthorship W3037844516A5061679017 @default.
- W3037844516 hasAuthorship W3037844516A5062223587 @default.
- W3037844516 hasAuthorship W3037844516A5064175599 @default.
- W3037844516 hasAuthorship W3037844516A5083564868 @default.
- W3037844516 hasBestOaLocation W30378445161 @default.
- W3037844516 hasConcept C177779419 @default.
- W3037844516 hasConcept C184235292 @default.
- W3037844516 hasConcept C185592680 @default.
- W3037844516 hasConcept C2780124434 @default.
- W3037844516 hasConcept C51911345 @default.
- W3037844516 hasConcept C62478195 @default.
- W3037844516 hasConcept C86803240 @default.
- W3037844516 hasConcept C95444343 @default.
- W3037844516 hasConcept C97029542 @default.
- W3037844516 hasConceptScore W3037844516C177779419 @default.
- W3037844516 hasConceptScore W3037844516C184235292 @default.