FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037923882> ?p ?o ?g. }

• W3037923882 endingPage "e04301" @default.
• W3037923882 startingPage "e04301" @default.
• W3037923882 abstract "Vaccination is one of the most effective interventions for preventing the spread of influenza viruses at the population level. Currently most influenza vaccines are produced by using embryonated chicken eggs, but alternative methods that achieve more rapid large-scale production are highly desirable for vaccines against both pandemic and seasonal influenza viruses. The use of recombinant hemagglutinin (HA), a key virus surface protein, as an antigen is an attractive candidate alternative approach, because of the potential for high protein yields and the ease of cloning new antigenic variants. Although fusion of HA with trimerization domains is needed to stabilize the trimeric structure and enhance the immunogenicity of the recombinant HA protein, whether the trimerization domains are immunogenic must be considered. Here, we generated recombinant multimeric HA without trimerization domains by using a short peptide linker, termed a single-chain HA (scHA), and evaluated scHAs as potential antigens for generating vaccines against influenza virus. Using mammalian cells, we succeeded in making three types of recombinant scHAs—two dimeric scHAs and a trimeric scHA. After immunization with aluminium salts in mice, one of the dimeric scHAs induced the greatest HA-specific IgG response among the scHAs and protected against virus challenge as strongly as the typically used trimeric HA containing a trimerization domain. We did not observe IgGs specific for the short peptide linker in mice immunized with the dimeric scHA, although IgGs specific for the trimerization domain occurred in mice immunized with the trimeric HA containing that domain. Furthermore, changing to another adjuvant did not diminish the utility of the dimeric scHA. These results suggest the potential usefulness of dimeric scHA as a vaccine antigen. We believe that single-chain antigens may represent new alternatives for production of recombinant antigen–based vaccines." @default.
• W3037923882 created "2020-07-02" @default.
• W3037923882 creator A5018328802 @default.
• W3037923882 creator A5025741589 @default.
• W3037923882 creator A5032281643 @default.
• W3037923882 date "2020-06-01" @default.
• W3037923882 modified "2023-10-16" @default.
• W3037923882 title "Vaccine effect of recombinant single-chain hemagglutinin protein as an antigen" @default.
• W3037923882 cites W1496645167 @default.
• W3037923882 cites W1984024271 @default.
• W3037923882 cites W1990545448 @default.
• W3037923882 cites W2006840424 @default.
• W3037923882 cites W2035217221 @default.
• W3037923882 cites W2058343458 @default.
• W3037923882 cites W2060880470 @default.
• W3037923882 cites W2071629929 @default.
• W3037923882 cites W2095336525 @default.
• W3037923882 cites W2097314439 @default.
• W3037923882 cites W2104391453 @default.
• W3037923882 cites W2105225614 @default.
• W3037923882 cites W2105841559 @default.
• W3037923882 cites W2116555540 @default.
• W3037923882 cites W2117430529 @default.
• W3037923882 cites W2126114330 @default.
• W3037923882 cites W2130737480 @default.
• W3037923882 cites W2134599862 @default.
• W3037923882 cites W2153541811 @default.
• W3037923882 cites W2269265106 @default.
• W3037923882 cites W2274976235 @default.
• W3037923882 cites W2464338232 @default.
• W3037923882 cites W2529384825 @default.
• W3037923882 cites W2576924838 @default.
• W3037923882 cites W2710124253 @default.
• W3037923882 cites W2737480209 @default.
• W3037923882 cites W2738554310 @default.
• W3037923882 cites W2800687120 @default.
• W3037923882 cites W2872545267 @default.
• W3037923882 doi "https://doi.org/10.1016/j.heliyon.2020.e04301" @default.
• W3037923882 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7327749" @default.
• W3037923882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32637694" @default.
• W3037923882 hasPublicationYear "2020" @default.
• W3037923882 type Work @default.
• W3037923882 sameAs 3037923882 @default.
• W3037923882 citedByCount "2" @default.
• W3037923882 countsByYear W30379238822021 @default.
• W3037923882 crossrefType "journal-article" @default.
• W3037923882 hasAuthorship W3037923882A5018328802 @default.
• W3037923882 hasAuthorship W3037923882A5025741589 @default.
• W3037923882 hasAuthorship W3037923882A5032281643 @default.
• W3037923882 hasBestOaLocation W30379238821 @default.
• W3037923882 hasConcept C104317684 @default.
• W3037923882 hasConcept C147483822 @default.
• W3037923882 hasConcept C159047783 @default.
• W3037923882 hasConcept C2522874641 @default.
• W3037923882 hasConcept C2777546802 @default.
• W3037923882 hasConcept C2778692840 @default.
• W3037923882 hasConcept C2780868878 @default.
• W3037923882 hasConcept C40767141 @default.
• W3037923882 hasConcept C54355233 @default.
• W3037923882 hasConcept C55493867 @default.
• W3037923882 hasConcept C86803240 @default.
• W3037923882 hasConceptScore W3037923882C104317684 @default.
• W3037923882 hasConceptScore W3037923882C147483822 @default.
• W3037923882 hasConceptScore W3037923882C159047783 @default.
• W3037923882 hasConceptScore W3037923882C2522874641 @default.
• W3037923882 hasConceptScore W3037923882C2777546802 @default.
• W3037923882 hasConceptScore W3037923882C2778692840 @default.
• W3037923882 hasConceptScore W3037923882C2780868878 @default.
• W3037923882 hasConceptScore W3037923882C40767141 @default.
• W3037923882 hasConceptScore W3037923882C54355233 @default.
• W3037923882 hasConceptScore W3037923882C55493867 @default.
• W3037923882 hasConceptScore W3037923882C86803240 @default.
• W3037923882 hasFunder F4320334764 @default.
• W3037923882 hasIssue "6" @default.
• W3037923882 hasLocation W30379238821 @default.
• W3037923882 hasLocation W30379238822 @default.
• W3037923882 hasLocation W30379238823 @default.
• W3037923882 hasLocation W30379238824 @default.
• W3037923882 hasOpenAccess W3037923882 @default.
• W3037923882 hasPrimaryLocation W30379238821 @default.
• W3037923882 hasRelatedWork W1024660077 @default.
• W3037923882 hasRelatedWork W2061818659 @default.
• W3037923882 hasRelatedWork W2071322149 @default.
• W3037923882 hasRelatedWork W2088538321 @default.
• W3037923882 hasRelatedWork W2107100610 @default.
• W3037923882 hasRelatedWork W2204875792 @default.
• W3037923882 hasRelatedWork W2316263092 @default.
• W3037923882 hasRelatedWork W2339595363 @default.
• W3037923882 hasRelatedWork W2413665266 @default.
• W3037923882 hasRelatedWork W4289779535 @default.
• W3037923882 hasVolume "6" @default.
• W3037923882 isParatext "false" @default.
• W3037923882 isRetracted "false" @default.
• W3037923882 magId "3037923882" @default.
• W3037923882 workType "article" @default.