Matches in SemOpenAlex for { <https://semopenalex.org/work/W3037930147> ?p ?o ?g. }
- W3037930147 endingPage "953" @default.
- W3037930147 startingPage "941" @default.
- W3037930147 abstract "The dengue virus (DENV) replication complex is made up of its non-structural (NS) proteins and yet-to-be identified host proteins, but the molecular interactions between these proteins are not fully elucidated. In this work, we sought to uncover the interactions between DENV NS1 and its fellow NS proteins using a yeast two-hybrid (Y2H) approach, and found that domain II of NS1 binds to an N-terminal cytoplasmic fragment of NS4A. Mutations in amino acid residues 41 and 43 in this cytoplasmic region of NS4A disrupted the interaction between NS1 and the NS4A-2K-4B precursor protein. When the NS4A Y41F mutation was introduced into the context of the virus via a DENV2 infectious clone, this mutant virus exhibited impaired viral fitness and decreased infectious virus production. The NS4A Y41F mutant virus triggered a significantly muted transcriptional activation of interferon-stimulated genes compared to wild-type virus that is independent of NS4A’s ability to antagonize type I interferon signalling. Taken together, we have identified a link between DENV NS1 and the cytoplasmic domain in NS4A that is important for its cellular and viral functions." @default.
- W3037930147 created "2020-07-02" @default.
- W3037930147 creator A5043393709 @default.
- W3037930147 creator A5065527770 @default.
- W3037930147 creator A5071946815 @default.
- W3037930147 creator A5085782731 @default.
- W3037930147 creator A5088498054 @default.
- W3037930147 creator A5090376613 @default.
- W3037930147 date "2020-09-01" @default.
- W3037930147 modified "2023-10-16" @default.
- W3037930147 title "Mutations in the cytoplasmic domain of dengue virus NS4A affect virus fitness and interactions with other non-structural proteins" @default.
- W3037930147 cites W1770656784 @default.
- W3037930147 cites W1964377967 @default.
- W3037930147 cites W1975050610 @default.
- W3037930147 cites W1980482308 @default.
- W3037930147 cites W1983907306 @default.
- W3037930147 cites W2008216856 @default.
- W3037930147 cites W2012551953 @default.
- W3037930147 cites W2016579684 @default.
- W3037930147 cites W2018082743 @default.
- W3037930147 cites W2021210077 @default.
- W3037930147 cites W2024970900 @default.
- W3037930147 cites W2025301385 @default.
- W3037930147 cites W2029052029 @default.
- W3037930147 cites W2051355772 @default.
- W3037930147 cites W2053850578 @default.
- W3037930147 cites W2061241820 @default.
- W3037930147 cites W2063401103 @default.
- W3037930147 cites W2066713215 @default.
- W3037930147 cites W2071225095 @default.
- W3037930147 cites W2083720374 @default.
- W3037930147 cites W2086858277 @default.
- W3037930147 cites W2103468489 @default.
- W3037930147 cites W2109788141 @default.
- W3037930147 cites W2113500493 @default.
- W3037930147 cites W2113967550 @default.
- W3037930147 cites W2119473061 @default.
- W3037930147 cites W2119525935 @default.
- W3037930147 cites W2131222241 @default.
- W3037930147 cites W2142481964 @default.
- W3037930147 cites W2145259841 @default.
- W3037930147 cites W2150029541 @default.
- W3037930147 cites W2161552841 @default.
- W3037930147 cites W2166771618 @default.
- W3037930147 cites W2178932929 @default.
- W3037930147 cites W2302414979 @default.
- W3037930147 cites W2326654974 @default.
- W3037930147 cites W2520747219 @default.
- W3037930147 cites W2763801778 @default.
- W3037930147 cites W2766221612 @default.
- W3037930147 cites W2806516470 @default.
- W3037930147 cites W2944745527 @default.
- W3037930147 cites W2954690080 @default.
- W3037930147 cites W4211135732 @default.
- W3037930147 doi "https://doi.org/10.1099/jgv.0.001462" @default.
- W3037930147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32589122" @default.
- W3037930147 hasPublicationYear "2020" @default.
- W3037930147 type Work @default.
- W3037930147 sameAs 3037930147 @default.
- W3037930147 citedByCount "10" @default.
- W3037930147 countsByYear W30379301472021 @default.
- W3037930147 countsByYear W30379301472022 @default.
- W3037930147 countsByYear W30379301472023 @default.
- W3037930147 crossrefType "journal-article" @default.
- W3037930147 hasAuthorship W3037930147A5043393709 @default.
- W3037930147 hasAuthorship W3037930147A5065527770 @default.
- W3037930147 hasAuthorship W3037930147A5071946815 @default.
- W3037930147 hasAuthorship W3037930147A5085782731 @default.
- W3037930147 hasAuthorship W3037930147A5088498054 @default.
- W3037930147 hasAuthorship W3037930147A5090376613 @default.
- W3037930147 hasBestOaLocation W30379301471 @default.
- W3037930147 hasConcept C104317684 @default.
- W3037930147 hasConcept C140704245 @default.
- W3037930147 hasConcept C143065580 @default.
- W3037930147 hasConcept C159047783 @default.
- W3037930147 hasConcept C190062978 @default.
- W3037930147 hasConcept C2522874641 @default.
- W3037930147 hasConcept C2776178377 @default.
- W3037930147 hasConcept C2779635636 @default.
- W3037930147 hasConcept C501734568 @default.
- W3037930147 hasConcept C533803919 @default.
- W3037930147 hasConcept C54355233 @default.
- W3037930147 hasConcept C86803240 @default.
- W3037930147 hasConceptScore W3037930147C104317684 @default.
- W3037930147 hasConceptScore W3037930147C140704245 @default.
- W3037930147 hasConceptScore W3037930147C143065580 @default.
- W3037930147 hasConceptScore W3037930147C159047783 @default.
- W3037930147 hasConceptScore W3037930147C190062978 @default.
- W3037930147 hasConceptScore W3037930147C2522874641 @default.
- W3037930147 hasConceptScore W3037930147C2776178377 @default.
- W3037930147 hasConceptScore W3037930147C2779635636 @default.
- W3037930147 hasConceptScore W3037930147C501734568 @default.
- W3037930147 hasConceptScore W3037930147C533803919 @default.
- W3037930147 hasConceptScore W3037930147C54355233 @default.
- W3037930147 hasConceptScore W3037930147C86803240 @default.
- W3037930147 hasFunder F4320334737 @default.
- W3037930147 hasIssue "9" @default.
- W3037930147 hasLocation W30379301471 @default.