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- W3038148146 abstract "Ciliary neurotrophic factor (CNTF) is one of the most studied neuroprotective agents with acknowledged potential in treating diseases of the posterior eye segment. Although its efficacy and mechanisms of action in the retina have been studied extensively, it is still not comprehensively understood which retinal cells mediate the therapeutic effects of CNTF. As with therapeutic proteins in general, it is poorly elucidated whether exogenous CNTF administered into the vitreous can enter and distribute into the retina and hence reach potentially responsive target cells. Here, we have characterized our purified recombinant human CNTF (rhCNTF), studied the protein’s in vitro bioactivity in a cell-based assay, and evaluated the thermodynamic and oligomeric status of the protein during storage. Biological activity of rhCNTF was further evaluated in vivo in an animal model of retinal degeneration. The retinal penetration and distribution of rhCNTF after 24 h was studied utilizing two ex vivo retina models. Based on our characterization findings, our rhCNTF is correctly folded and biologically active. Moreover, based on initial screening and subsequent follow-up, we identified two buffers in which rhCNTF retains its stability during storage. Whereas rhCNTF did not show photoreceptor preservative effect or improve the function of photoreceptors in vivo, this could possibly be due to the used disease model or the short duration of action with a single intravitreal injection of rhCNTF. On the other hand, the lack of in vivo efficacy was shown to not be due to distribution limitations; permeation into the retina was observed in both retinal explant models as in 24 h rhCNTF penetrated the inner limiting membrane, and being mostly observed in the ganglion cell layer, distributed to different layers of the neural retina. As rhCNTF can reach deeper retinal layers, in general, having direct effects on resident CNTF-responsive target cells is plausible." @default.
- W3038148146 created "2020-07-10" @default.
- W3038148146 creator A5000387665 @default.
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- W3038148146 creator A5091424033 @default.
- W3038148146 date "2020-06-30" @default.
- W3038148146 modified "2023-10-14" @default.
- W3038148146 title "Characterization, Stability, and In Vivo Efficacy Studies of Recombinant Human CNTF and Its Permeation into the Neural Retina in Ex Vivo Organotypic Retinal Explant Culture Models" @default.
- W3038148146 cites W1493793044 @default.
- W3038148146 cites W1499757659 @default.
- W3038148146 cites W1506004290 @default.
- W3038148146 cites W1562834221 @default.
- W3038148146 cites W1588979652 @default.
- W3038148146 cites W1597900071 @default.
- W3038148146 cites W1776302394 @default.
- W3038148146 cites W1780664938 @default.
- W3038148146 cites W1810883461 @default.
- W3038148146 cites W1861582625 @default.
- W3038148146 cites W1888897387 @default.
- W3038148146 cites W1964211606 @default.
- W3038148146 cites W1971174028 @default.
- W3038148146 cites W1972955056 @default.
- W3038148146 cites W1974084948 @default.
- W3038148146 cites W1977683311 @default.
- W3038148146 cites W1981842396 @default.
- W3038148146 cites W1984857940 @default.
- W3038148146 cites W1986527511 @default.
- W3038148146 cites W1988233478 @default.
- W3038148146 cites W1991909711 @default.
- W3038148146 cites W1995221631 @default.
- W3038148146 cites W1997410081 @default.
- W3038148146 cites W2001141741 @default.
- W3038148146 cites W2001272091 @default.
- W3038148146 cites W2005088734 @default.
- W3038148146 cites W2005372537 @default.
- W3038148146 cites W2006434448 @default.
- W3038148146 cites W2013215292 @default.
- W3038148146 cites W2021340181 @default.
- W3038148146 cites W2025938842 @default.
- W3038148146 cites W2027513152 @default.
- W3038148146 cites W2027788764 @default.
- W3038148146 cites W2033915545 @default.
- W3038148146 cites W2034571018 @default.
- W3038148146 cites W2035585391 @default.
- W3038148146 cites W2039936409 @default.
- W3038148146 cites W2041099386 @default.
- W3038148146 cites W2041294595 @default.
- W3038148146 cites W2048881497 @default.
- W3038148146 cites W2049484831 @default.
- W3038148146 cites W2050384954 @default.
- W3038148146 cites W2052113794 @default.
- W3038148146 cites W2059323909 @default.
- W3038148146 cites W2059500485 @default.
- W3038148146 cites W2062885086 @default.
- W3038148146 cites W2070130037 @default.
- W3038148146 cites W2070403068 @default.
- W3038148146 cites W2077203674 @default.
- W3038148146 cites W2082060456 @default.
- W3038148146 cites W2084959174 @default.
- W3038148146 cites W2085983277 @default.
- W3038148146 cites W2087303190 @default.
- W3038148146 cites W2088651355 @default.
- W3038148146 cites W2094688362 @default.
- W3038148146 cites W2096993232 @default.
- W3038148146 cites W2106246537 @default.
- W3038148146 cites W2113920016 @default.
- W3038148146 cites W2127519720 @default.
- W3038148146 cites W2142761289 @default.
- W3038148146 cites W2147974112 @default.
- W3038148146 cites W2155747723 @default.
- W3038148146 cites W2157508553 @default.
- W3038148146 cites W2159254906 @default.
- W3038148146 cites W2159423768 @default.
- W3038148146 cites W2161045200 @default.
- W3038148146 cites W2161994209 @default.
- W3038148146 cites W2168681404 @default.
- W3038148146 cites W2227187219 @default.
- W3038148146 cites W2315368953 @default.
- W3038148146 cites W2324172839 @default.
- W3038148146 cites W2327032806 @default.
- W3038148146 cites W2345334620 @default.
- W3038148146 cites W2410466918 @default.
- W3038148146 cites W2413607750 @default.
- W3038148146 cites W2460478662 @default.
- W3038148146 cites W2505021214 @default.
- W3038148146 cites W2522303657 @default.
- W3038148146 cites W2528451823 @default.
- W3038148146 cites W2551905882 @default.
- W3038148146 cites W2553402145 @default.
- W3038148146 cites W2560243645 @default.
- W3038148146 cites W2567416294 @default.
- W3038148146 cites W2595579507 @default.