FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3038210189> ?p ?o ?g. }

• W3038210189 abstract "Optogenetics offers unprecedented possibilities to investigate cortical networks. Yet, the number of successful optogenetic applications in non-human primates is still low, and the consequences of opsin expression in the primate brain are not well documented. We assessed histologically if we can target cerebrocortical networks with three common optogenetic constructs (AAV2/5-CaMKIIα-eNpHR3.0-mCherry, -ChR2-eYFP, -C1V1-mCherry). The frontal eye field or the dorsal premotor area of rhesus macaques were virally injected, and the resulting transduction spread, expression specificity, and opsin trafficking into axons projecting to parietal and visual areas were examined. After variable periods (2-24 months), expression was robust for all constructs at the injection sites. The CaMKIIα promoter driven-expression was predominant, but not exclusive, in excitatory neurons. In the case of eNpHR3.0-mCherry and ChR2-eYFP, opsins were present in axonal projections to target areas, in which sparse, retrogradely transduced neurons could also be found. Finally, the intracellular distribution of opsins differed: ChR2-eYFP had almost exclusive membrane localization, while eNpHR3.0-mCherry and C1V1-mCherry showed additional intracellular accumulations, which might affect neuronal survival in the long-term. Results indicate that all three constructs can be used for local neuronal modulation, but axonal stimulation and long-term use require additional considerations of construct selection and verification." @default.
• W3038210189 created "2020-07-10" @default.
• W3038210189 creator A5008513913 @default.
• W3038210189 creator A5013266216 @default.
• W3038210189 creator A5019351076 @default.
• W3038210189 creator A5036806876 @default.
• W3038210189 creator A5037638886 @default.
• W3038210189 creator A5051705174 @default.
• W3038210189 creator A5066942106 @default.
• W3038210189 creator A5074586431 @default.
• W3038210189 creator A5087666437 @default.
• W3038210189 date "2020-07-06" @default.
• W3038210189 modified "2023-10-16" @default.
• W3038210189 title "Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque" @default.
• W3038210189 cites W1139015881 @default.
• W3038210189 cites W1599549671 @default.
• W3038210189 cites W1681317868 @default.
• W3038210189 cites W1838936138 @default.
• W3038210189 cites W1914605476 @default.
• W3038210189 cites W1970839656 @default.
• W3038210189 cites W1973468287 @default.
• W3038210189 cites W1975032719 @default.
• W3038210189 cites W1985804114 @default.
• W3038210189 cites W1985854422 @default.
• W3038210189 cites W1996778329 @default.
• W3038210189 cites W2003456064 @default.
• W3038210189 cites W2015741711 @default.
• W3038210189 cites W2023732600 @default.
• W3038210189 cites W2034325864 @default.
• W3038210189 cites W2040618303 @default.
• W3038210189 cites W2042656725 @default.
• W3038210189 cites W2046858324 @default.
• W3038210189 cites W2047627760 @default.
• W3038210189 cites W2060978874 @default.
• W3038210189 cites W2076139053 @default.
• W3038210189 cites W2091248412 @default.
• W3038210189 cites W2101238942 @default.
• W3038210189 cites W2108076489 @default.
• W3038210189 cites W2116537784 @default.
• W3038210189 cites W2117957894 @default.
• W3038210189 cites W2136993243 @default.
• W3038210189 cites W2139272195 @default.
• W3038210189 cites W2140206964 @default.
• W3038210189 cites W2146584976 @default.
• W3038210189 cites W2156692671 @default.
• W3038210189 cites W2158256695 @default.
• W3038210189 cites W2160779028 @default.
• W3038210189 cites W2170042605 @default.
• W3038210189 cites W2271615357 @default.
• W3038210189 cites W2284290157 @default.
• W3038210189 cites W2286473979 @default.
• W3038210189 cites W2305455886 @default.
• W3038210189 cites W2311993108 @default.
• W3038210189 cites W2327280257 @default.
• W3038210189 cites W2339207940 @default.
• W3038210189 cites W2345465587 @default.
• W3038210189 cites W2468113650 @default.
• W3038210189 cites W2503929286 @default.
• W3038210189 cites W2546661444 @default.
• W3038210189 cites W2588541268 @default.
• W3038210189 cites W2591571009 @default.
• W3038210189 cites W2596596521 @default.
• W3038210189 cites W2616038248 @default.
• W3038210189 cites W2768955185 @default.
• W3038210189 cites W2771198931 @default.
• W3038210189 cites W2780733918 @default.
• W3038210189 cites W2793042966 @default.
• W3038210189 cites W2800214089 @default.
• W3038210189 cites W2801454635 @default.
• W3038210189 cites W2807086437 @default.
• W3038210189 cites W2940481586 @default.
• W3038210189 cites W2949734859 @default.
• W3038210189 cites W2966815797 @default.
• W3038210189 cites W2975285816 @default.
• W3038210189 cites W2980650648 @default.
• W3038210189 cites W4210574564 @default.
• W3038210189 cites W4211034564 @default.
• W3038210189 cites W4239908405 @default.
• W3038210189 doi "https://doi.org/10.1038/s41598-020-67752-6" @default.
• W3038210189 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7338380" @default.
• W3038210189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32632196" @default.
• W3038210189 hasPublicationYear "2020" @default.
• W3038210189 type Work @default.
• W3038210189 sameAs 3038210189 @default.
• W3038210189 citedByCount "5" @default.
• W3038210189 countsByYear W30382101892020 @default.
• W3038210189 countsByYear W30382101892022 @default.
• W3038210189 countsByYear W30382101892023 @default.
• W3038210189 crossrefType "journal-article" @default.
• W3038210189 hasAuthorship W3038210189A5008513913 @default.
• W3038210189 hasAuthorship W3038210189A5013266216 @default.
• W3038210189 hasAuthorship W3038210189A5019351076 @default.
• W3038210189 hasAuthorship W3038210189A5036806876 @default.
• W3038210189 hasAuthorship W3038210189A5037638886 @default.
• W3038210189 hasAuthorship W3038210189A5051705174 @default.
• W3038210189 hasAuthorship W3038210189A5066942106 @default.
• W3038210189 hasAuthorship W3038210189A5074586431 @default.
• W3038210189 hasAuthorship W3038210189A5087666437 @default.
• W3038210189 hasBestOaLocation W30382101891 @default.
• W3038210189 hasConcept C104317684 @default.

• next