FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3038483669> ?p ?o ?g. }

• W3038483669 endingPage "1647" @default.
• W3038483669 startingPage "1637" @default.
• W3038483669 abstract "Abstract STUDY QUESTION Do supraphysiologic estradiol (E2) levels in the ranges attained during normal and high response superovulation cycles modify the onset of endometrial secretory transformation? SUMMARY ANSWER Highly supraphysiologic levels of E2 do not alter the ability of physiologic levels of progesterone (P4) to induce secretory transformation. WHAT IS KNOWN ALREADY Previous studies have demonstrated that premature P4 elevations during IVF cycles are associated with a decrement in clinical pregnancy rates after fresh embryo transfer due to shifts in the window of implantation (WOI). However, alterations in the onset of secretory transformation may not apply uniformly to all patients. High responders with supraphysiologic E2 levels accompanied by similar subtle increases in P4 have not been shown to have decreased sustained implantation rates. This prospective investigation in which whole-genome transcriptomic and methylomic analysis of the endometrium is performed for individual patients under a range of E2 concentrations brings clarity to a long-debated issue. STUDY DESIGN, SIZE, DURATION A randomized, prospective and paired trial was conducted in which 10 participants were enrolled and randomized to the order in which they completed three distinct uterine stimulation cycles, each at a specific E2 concentration: physiologic (∼180 pg/ml), moderately supraphysiologic (600–800 pg/ml) or supraphysiologic (2000 pg/ml). Target E2 ranges were selected to mimic those seen in natural, controlled ovarian stimulation and IVF cycles. E2 valerate was administered in order to maintain stable E2 levels for 12 days followed by intramuscular P4 in oil 10 mg/day for two doses, after which an endometrial biopsy was performed. A total of 30 endometrial biopsies were included in a whole-genome transcriptomic and methylomic analysis. PARTICIPANTS/MATERIALS, SETTING, METHODS Healthy volunteers without a history of infertility were included in this study at a single large infertility center. DNA was isolated from the endometrial biopsy specimens and bisulfite sequencing was performed to construct a methylation array. Differential methylation analysis was conducted based on differences in M-values of individuals across treatment groups for each probe as well as carrying out t-tests. RNA was isolated for RNA-Seq analysis and gene expression values were compared using DESeq2. All analyses were performed in a pairwise fashion to compare among the three stimulation cycles within individuals and secondarily to compare all participants in each of the cycles. MAIN RESULTS AND THE ROLE OF CHANCE The mean peak E2 and P4 levels were 275 pg/ml and 4.17 ng/ml in the physiologic group, 910 pg/ml and 2.69 ng/ml in the moderate group was, and 2043 pg/ml and 2.64 ng/ml in the supraphysiologic group, respectively. Principal component analysis of 834 913 CpG sites was performed on M-values of individuals within the low, moderate and supraphysiologic conditions in a paired approach. There were no differences in genome-wide methylation within participants across E2 groups. A paired analysis revealed that gene expression profiles did not differ within the same individual at each of the three E2 levels. No significant alterations in gene expression as related to endometrial physiology were identified between the low, moderate and supraphysiologic groups in an inter-participant analysis. LIMITATIONS, REASONS FOR CAUTION Although each participant completed a physiologic cycle in which E2 levels were maintained in a range that would simulate a natural cycle, our findings are limited by lack of an unmedicated control to assess if there was a potential effect from E2V. Additionally, our results were obtained in fertile individuals, who may have a different endometrial response compared to an infertile population. Despite the whole genomic endometrial assessment and rigorous, paired study design, the sample size was limited. WIDER IMPLICATIONS OF THE FINDINGS Given that the endometrial response to P4 is unaffected by E2 levels in the supraphysiologic range, diminutions in implantation seen in stimulated cycles may result from embryonic-endometrial dyssynchrony following early P4 elevations or slowly blastulating embryos, which occur independently of the magnitude of the E2 rise. STUDY FUNDING/COMPETING INTEREST(S) This study was funded by the Foundation for Embryonic Competence, Basking Ridge, NJ, USA. Dr E.S. reports consultancy work for The Foundation for Embryonic Competence, Basking Ridge, NJ, USA. The other authors declare no conflict of interests related to this topic. TRIAL REGISTRATION NUMBER NCT02458404." @default.
• W3038483669 created "2020-07-10" @default.
• W3038483669 creator A5011160568 @default.
• W3038483669 creator A5027425789 @default.
• W3038483669 creator A5051584022 @default.
• W3038483669 creator A5062847244 @default.
• W3038483669 creator A5073396332 @default.
• W3038483669 creator A5079490860 @default.
• W3038483669 creator A5080335472 @default.
• W3038483669 creator A5090198196 @default.
• W3038483669 date "2020-07-01" @default.
• W3038483669 modified "2023-10-11" @default.
• W3038483669 title "Varying levels of serum estradiol do not alter the timing of the early endometrial secretory transformation" @default.
• W3038483669 cites W1482295836 @default.
• W3038483669 cites W1884252026 @default.
• W3038483669 cites W1964021013 @default.
• W3038483669 cites W1964764413 @default.
• W3038483669 cites W1965676721 @default.
• W3038483669 cites W1975833969 @default.
• W3038483669 cites W1992001268 @default.
• W3038483669 cites W1994993513 @default.
• W3038483669 cites W1999516495 @default.
• W3038483669 cites W2000886639 @default.
• W3038483669 cites W2047700804 @default.
• W3038483669 cites W2061036594 @default.
• W3038483669 cites W2069362033 @default.
• W3038483669 cites W2070108578 @default.
• W3038483669 cites W2072617630 @default.
• W3038483669 cites W2078327537 @default.
• W3038483669 cites W2081182312 @default.
• W3038483669 cites W2088267656 @default.
• W3038483669 cites W2088469017 @default.
• W3038483669 cites W2092394511 @default.
• W3038483669 cites W2101870168 @default.
• W3038483669 cites W2107755789 @default.
• W3038483669 cites W2110160605 @default.
• W3038483669 cites W2116774269 @default.
• W3038483669 cites W2127915467 @default.
• W3038483669 cites W2139670900 @default.
• W3038483669 cites W2153214140 @default.
• W3038483669 cites W2156292936 @default.
• W3038483669 cites W2161479512 @default.
• W3038483669 cites W2167852330 @default.
• W3038483669 cites W2179438025 @default.
• W3038483669 cites W2292087464 @default.
• W3038483669 cites W2325674170 @default.
• W3038483669 cites W2411205054 @default.
• W3038483669 cites W2510875395 @default.
• W3038483669 cites W2513803129 @default.
• W3038483669 cites W2582792555 @default.
• W3038483669 cites W2598378902 @default.
• W3038483669 cites W2624413373 @default.
• W3038483669 cites W2710353577 @default.
• W3038483669 cites W2739638102 @default.
• W3038483669 cites W2741972452 @default.
• W3038483669 cites W2743164141 @default.
• W3038483669 cites W2794229676 @default.
• W3038483669 cites W2898598946 @default.
• W3038483669 cites W2916530067 @default.
• W3038483669 cites W2993782226 @default.
• W3038483669 cites W4243922158 @default.
• W3038483669 doi "https://doi.org/10.1093/humrep/deaa135" @default.
• W3038483669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32613240" @default.
• W3038483669 hasPublicationYear "2020" @default.
• W3038483669 type Work @default.
• W3038483669 sameAs 3038483669 @default.
• W3038483669 citedByCount "4" @default.
• W3038483669 countsByYear W30384836692021 @default.
• W3038483669 countsByYear W30384836692022 @default.
• W3038483669 countsByYear W30384836692023 @default.
• W3038483669 crossrefType "journal-article" @default.
• W3038483669 hasAuthorship W3038483669A5011160568 @default.
• W3038483669 hasAuthorship W3038483669A5027425789 @default.
• W3038483669 hasAuthorship W3038483669A5051584022 @default.
• W3038483669 hasAuthorship W3038483669A5062847244 @default.
• W3038483669 hasAuthorship W3038483669A5073396332 @default.
• W3038483669 hasAuthorship W3038483669A5079490860 @default.
• W3038483669 hasAuthorship W3038483669A5080335472 @default.
• W3038483669 hasAuthorship W3038483669A5090198196 @default.
• W3038483669 hasBestOaLocation W30384836691 @default.
• W3038483669 hasConcept C126322002 @default.
• W3038483669 hasConcept C134018914 @default.
• W3038483669 hasConcept C160099875 @default.
• W3038483669 hasConcept C16685009 @default.
• W3038483669 hasConcept C188816634 @default.
• W3038483669 hasConcept C24998067 @default.
• W3038483669 hasConcept C2776606343 @default.
• W3038483669 hasConcept C2777688143 @default.
• W3038483669 hasConcept C2779234561 @default.
• W3038483669 hasConcept C2779742232 @default.
• W3038483669 hasConcept C54355233 @default.
• W3038483669 hasConcept C71924100 @default.
• W3038483669 hasConcept C86803240 @default.
• W3038483669 hasConceptScore W3038483669C126322002 @default.
• W3038483669 hasConceptScore W3038483669C134018914 @default.
• W3038483669 hasConceptScore W3038483669C160099875 @default.
• W3038483669 hasConceptScore W3038483669C16685009 @default.
• W3038483669 hasConceptScore W3038483669C188816634 @default.

• next