FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3038520299> ?p ?o ?g. }

• W3038520299 endingPage "5312" @default.
• W3038520299 startingPage "5302" @default.
• W3038520299 abstract "Abstract Rubisco is central to carbon assimilation, and efforts to improve the efficiency and sustainability of crop production have spurred interest in phenotyping Rubisco activity. We tested the hypothesis that microtiter plate-based methods provide comparable results to those obtained with the radiometric assay that measures the incorporation of 14CO2 into 3-phosphoglycerate (3-PGA). Three NADH-linked assays were tested that use alternative coupling enzymes: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glycerolphosphate dehydrogenase (GlyPDH); phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH); and pyruvate kinase (PK) and lactate dehydrogenase (LDH). To date there has been no thorough evaluation of their reliability by comparison with the 14C-based method. The three NADH-linked assays were used in parallel to estimate (i) the 3-PGA concentration–response curve of NADH oxidation, (ii) the Michaelis–Menten constant for ribulose-1,5-bisphosphate, (iii) fully active and inhibited Rubisco activities, and (iv) Rubisco initial and total activities in fully illuminated and shaded leaves. All three methods correlated strongly with the 14C-based method, and the PK–LDH method showed a strong correlation and was the cheapest method. PEPC–MDH would be a suitable option for situations in which ADP/ATP might interfere with the assay. GAPDH–GlyPDH proved more laborious than the other methods. Thus, we recommend the PK–LDH method as a reliable, cheaper, and higher throughput method to phenotype Rubisco activity for crop improvement efforts." @default.
• W3038520299 created "2020-07-10" @default.
• W3038520299 creator A5066604368 @default.
• W3038520299 creator A5071579752 @default.
• W3038520299 creator A5089477042 @default.
• W3038520299 date "2020-06-30" @default.
• W3038520299 modified "2023-10-18" @default.
• W3038520299 title "Measuring Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays" @default.
• W3038520299 cites W132126081 @default.
• W3038520299 cites W1538776818 @default.
• W3038520299 cites W1606884205 @default.
• W3038520299 cites W1675201170 @default.
• W3038520299 cites W1990797677 @default.
• W3038520299 cites W2005793515 @default.
• W3038520299 cites W2012553720 @default.
• W3038520299 cites W2022087726 @default.
• W3038520299 cites W2026130119 @default.
• W3038520299 cites W2036102895 @default.
• W3038520299 cites W2039132912 @default.
• W3038520299 cites W2051553371 @default.
• W3038520299 cites W2051942624 @default.
• W3038520299 cites W2052599370 @default.
• W3038520299 cites W2053120694 @default.
• W3038520299 cites W2055636146 @default.
• W3038520299 cites W2057237418 @default.
• W3038520299 cites W2062899870 @default.
• W3038520299 cites W2075001104 @default.
• W3038520299 cites W2076026864 @default.
• W3038520299 cites W2104526954 @default.
• W3038520299 cites W2112164915 @default.
• W3038520299 cites W2116459611 @default.
• W3038520299 cites W2118476033 @default.
• W3038520299 cites W2121045266 @default.
• W3038520299 cites W2132174235 @default.
• W3038520299 cites W2139437870 @default.
• W3038520299 cites W2141909379 @default.
• W3038520299 cites W2145952050 @default.
• W3038520299 cites W2149381696 @default.
• W3038520299 cites W2212640525 @default.
• W3038520299 cites W2271743304 @default.
• W3038520299 cites W2299419053 @default.
• W3038520299 cites W2315768741 @default.
• W3038520299 cites W2343690410 @default.
• W3038520299 cites W2405747428 @default.
• W3038520299 cites W2470090207 @default.
• W3038520299 cites W2558604538 @default.
• W3038520299 cites W2560694993 @default.
• W3038520299 cites W2599999920 @default.
• W3038520299 cites W2707582342 @default.
• W3038520299 cites W2912682509 @default.
• W3038520299 cites W2950086965 @default.
• W3038520299 cites W2970208715 @default.
• W3038520299 cites W333539686 @default.
• W3038520299 doi "https://doi.org/10.1093/jxb/eraa289" @default.
• W3038520299 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7501812" @default.
• W3038520299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32728715" @default.
• W3038520299 hasPublicationYear "2020" @default.
• W3038520299 type Work @default.
• W3038520299 sameAs 3038520299 @default.
• W3038520299 citedByCount "11" @default.
• W3038520299 countsByYear W30385202992020 @default.
• W3038520299 countsByYear W30385202992021 @default.
• W3038520299 countsByYear W30385202992022 @default.
• W3038520299 crossrefType "journal-article" @default.
• W3038520299 hasAuthorship W3038520299A5066604368 @default.
• W3038520299 hasAuthorship W3038520299A5071579752 @default.
• W3038520299 hasAuthorship W3038520299A5089477042 @default.
• W3038520299 hasBestOaLocation W30385202991 @default.
• W3038520299 hasConcept C111341932 @default.
• W3038520299 hasConcept C121332964 @default.
• W3038520299 hasConcept C132901563 @default.
• W3038520299 hasConcept C134792803 @default.
• W3038520299 hasConcept C140137476 @default.
• W3038520299 hasConcept C181199279 @default.
• W3038520299 hasConcept C185592680 @default.
• W3038520299 hasConcept C2776317432 @default.
• W3038520299 hasConcept C2777318727 @default.
• W3038520299 hasConcept C2778861558 @default.
• W3038520299 hasConcept C2779966244 @default.
• W3038520299 hasConcept C50212798 @default.
• W3038520299 hasConcept C55493867 @default.
• W3038520299 hasConcept C62520636 @default.
• W3038520299 hasConcept C86803240 @default.
• W3038520299 hasConcept C91881484 @default.
• W3038520299 hasConceptScore W3038520299C111341932 @default.
• W3038520299 hasConceptScore W3038520299C121332964 @default.
• W3038520299 hasConceptScore W3038520299C132901563 @default.
• W3038520299 hasConceptScore W3038520299C134792803 @default.
• W3038520299 hasConceptScore W3038520299C140137476 @default.
• W3038520299 hasConceptScore W3038520299C181199279 @default.
• W3038520299 hasConceptScore W3038520299C185592680 @default.
• W3038520299 hasConceptScore W3038520299C2776317432 @default.
• W3038520299 hasConceptScore W3038520299C2777318727 @default.
• W3038520299 hasConceptScore W3038520299C2778861558 @default.
• W3038520299 hasConceptScore W3038520299C2779966244 @default.
• W3038520299 hasConceptScore W3038520299C50212798 @default.
• W3038520299 hasConceptScore W3038520299C55493867 @default.
• W3038520299 hasConceptScore W3038520299C62520636 @default.

• next