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• W3038694712 startingPage "910" @default.
• W3038694712 abstract "Accurate mapping of transcription start sites (TSSs) is key for understanding transcriptional regulation. However, current protocols for genome-wide TSS profiling are laborious and/or expensive. We present Survey of TRanscription Initiation at Promoter Elements with high-throughput sequencing (STRIPE-seq), a simple, rapid, and cost-effective protocol for sequencing capped RNA 5′ ends from as little as 50 ng total RNA. Including depletion of uncapped RNA and reaction cleanups, a STRIPE-seq library can be constructed in about 5 h. We show application of STRIPE-seq to TSS profiling in yeast and human cells and show that it can also be effectively used for quantification of transcript levels and analysis of differential gene expression. In conjunction with our ready-to-use computational workflows, STRIPE-seq is a straightforward, efficient means by which to probe the landscape of transcriptional initiation." @default.
• W3038694712 created "2020-07-10" @default.
• W3038694712 creator A5023435195 @default.
• W3038694712 creator A5033118239 @default.
• W3038694712 creator A5042741063 @default.
• W3038694712 creator A5073040813 @default.
• W3038694712 date "2020-06-01" @default.
• W3038694712 modified "2023-09-27" @default.
• W3038694712 title "Simple and efficient profiling of transcription initiation and transcript levels with STRIPE-seq" @default.
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• W3038694712 doi "https://doi.org/10.1101/gr.261545.120" @default.
• W3038694712 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7370879" @default.
• W3038694712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32660958" @default.
• W3038694712 hasPublicationYear "2020" @default.
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