Matches in SemOpenAlex for { <https://semopenalex.org/work/W3038897434> ?p ?o ?g. }
- W3038897434 endingPage "8320" @default.
- W3038897434 startingPage "8308" @default.
- W3038897434 abstract "Biologically active peptides in milk proteins can be used as effective dietary supplements for management of bone-associated issues including osteoporosis. A bioactive peptide derived from milk, viz. VLPVPQK/PepC, has been validated previously from our lab for its osteoanabolic action. In this study, we report 14 novel variants of PepC, designed in silico, based on the structure–activity relationship, aiming to enhance its osteogenic effect that holds tremendous therapeutic utility for bone-related injuries. PepC was computationally modified at seven positions of its original sequence, resulting in 14 modified synthetic peptides for functional predictions and in vitro assessment by comparative analysis of modified peptides by PepC for improved ability in osteogenic functional assays (proliferation potential, antioxidant ability, gene and protein expression, cytotoxic effect, bone mineralization) using calvarial osteoblasts. For most peptides with the highest Peptide7 response relative to PepC (p < 0.05), enhanced osteoanabolic response was observed. Further observations on Peptide7 have therefore been investigated in depth (qPCR, immunoblotting, LCMS/MS, and PCA analysis). Peptide7 displayed a rise in the expression of osteogenes (Osterix, Opg, Bmp2, and Runx2, p < 0.05) and protein (Runx2 and Bmp2, p < 0.05). Besides, LCMS/MS findings suggest Peptide7 escapes intestinal peptidases degradation. Experimental evidence supports an improved osteological reaction to newly modified peptides and hence exploitation in the preparation of functional foods or supplements." @default.
- W3038897434 created "2020-07-10" @default.
- W3038897434 creator A5028255054 @default.
- W3038897434 creator A5053070331 @default.
- W3038897434 creator A5066367042 @default.
- W3038897434 creator A5081487121 @default.
- W3038897434 date "2020-07-06" @default.
- W3038897434 modified "2023-09-24" @default.
- W3038897434 title "Repertoire of Structure–Activity-Based Novel Modified Peptides Elicits Enhanced Osteogenic Potential" @default.
- W3038897434 cites W103677414 @default.
- W3038897434 cites W1795935625 @default.
- W3038897434 cites W1985073259 @default.
- W3038897434 cites W1985547648 @default.
- W3038897434 cites W1986276800 @default.
- W3038897434 cites W1989153526 @default.
- W3038897434 cites W1994778261 @default.
- W3038897434 cites W1997868832 @default.
- W3038897434 cites W2006107863 @default.
- W3038897434 cites W2006276964 @default.
- W3038897434 cites W2019487320 @default.
- W3038897434 cites W2054189422 @default.
- W3038897434 cites W2061819518 @default.
- W3038897434 cites W2081277508 @default.
- W3038897434 cites W2109504045 @default.
- W3038897434 cites W2114918609 @default.
- W3038897434 cites W2117028405 @default.
- W3038897434 cites W2129535549 @default.
- W3038897434 cites W2136571121 @default.
- W3038897434 cites W2139055053 @default.
- W3038897434 cites W2143480235 @default.
- W3038897434 cites W2153234283 @default.
- W3038897434 cites W2160780417 @default.
- W3038897434 cites W2167734754 @default.
- W3038897434 cites W2182303898 @default.
- W3038897434 cites W2295124130 @default.
- W3038897434 cites W2308058717 @default.
- W3038897434 cites W2316245666 @default.
- W3038897434 cites W2398628007 @default.
- W3038897434 cites W2418518399 @default.
- W3038897434 cites W2465349585 @default.
- W3038897434 cites W2556125626 @default.
- W3038897434 cites W2559809466 @default.
- W3038897434 cites W2571735392 @default.
- W3038897434 cites W2591160490 @default.
- W3038897434 cites W2609244481 @default.
- W3038897434 cites W2790930653 @default.
- W3038897434 cites W2793401355 @default.
- W3038897434 cites W2796531554 @default.
- W3038897434 cites W2798087515 @default.
- W3038897434 cites W2802526399 @default.
- W3038897434 cites W2886022337 @default.
- W3038897434 cites W2899027927 @default.
- W3038897434 cites W2909713930 @default.
- W3038897434 cites W3007068541 @default.
- W3038897434 cites W3174642256 @default.
- W3038897434 cites W974254039 @default.
- W3038897434 doi "https://doi.org/10.1021/acs.jafc.0c03385" @default.
- W3038897434 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32628843" @default.
- W3038897434 hasPublicationYear "2020" @default.
- W3038897434 type Work @default.
- W3038897434 sameAs 3038897434 @default.
- W3038897434 citedByCount "5" @default.
- W3038897434 countsByYear W30388974342021 @default.
- W3038897434 countsByYear W30388974342022 @default.
- W3038897434 countsByYear W30388974342023 @default.
- W3038897434 crossrefType "journal-article" @default.
- W3038897434 hasAuthorship W3038897434A5028255054 @default.
- W3038897434 hasAuthorship W3038897434A5053070331 @default.
- W3038897434 hasAuthorship W3038897434A5066367042 @default.
- W3038897434 hasAuthorship W3038897434A5081487121 @default.
- W3038897434 hasConcept C104317684 @default.
- W3038897434 hasConcept C13935069 @default.
- W3038897434 hasConcept C185592680 @default.
- W3038897434 hasConcept C202751555 @default.
- W3038897434 hasConcept C2775905019 @default.
- W3038897434 hasConcept C2776363554 @default.
- W3038897434 hasConcept C2778260815 @default.
- W3038897434 hasConcept C2779281246 @default.
- W3038897434 hasConcept C55493867 @default.
- W3038897434 hasConceptScore W3038897434C104317684 @default.
- W3038897434 hasConceptScore W3038897434C13935069 @default.
- W3038897434 hasConceptScore W3038897434C185592680 @default.
- W3038897434 hasConceptScore W3038897434C202751555 @default.
- W3038897434 hasConceptScore W3038897434C2775905019 @default.
- W3038897434 hasConceptScore W3038897434C2776363554 @default.
- W3038897434 hasConceptScore W3038897434C2778260815 @default.
- W3038897434 hasConceptScore W3038897434C2779281246 @default.
- W3038897434 hasConceptScore W3038897434C55493867 @default.
- W3038897434 hasIssue "31" @default.
- W3038897434 hasLocation W30388974341 @default.
- W3038897434 hasOpenAccess W3038897434 @default.
- W3038897434 hasPrimaryLocation W30388974341 @default.
- W3038897434 hasRelatedWork W2015986807 @default.
- W3038897434 hasRelatedWork W2019480635 @default.
- W3038897434 hasRelatedWork W2038712462 @default.
- W3038897434 hasRelatedWork W2043576708 @default.
- W3038897434 hasRelatedWork W2108725809 @default.
- W3038897434 hasRelatedWork W2160248324 @default.