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- W3039069481 abstract "Dissolving microneedles (DMNs) are vital for the transdermal delivery of protein-based drugs due to their painless penetration, effective drug permeation, and safety characteristics. Thus, it is necessary to quantify the proteins encapsulated inside the DMNs. The classic BCA(c-BCA) assay is commonly employed for protein quantification. However, the presence of dextran (Dex, an excipient of DMNs) interferes with the quantification of ovalbumin (OVA) by the c-BCA assay, leading to erroneous results. Therefore, two new techniques, including modified BCA (m-BCA) and reversed-phase HPLC (RP-HPLC) were developed to accurately determine the protein concentrations in pharmaceutical formulations, compared with the c-BCA assay. The results showed that the m-BCA and RP-HPLC methods were reliable for protein quantification in DMNs, with linear responses for correlation coefficients of >0.99; the acceptable added samples recoveries of 100 ± 10 %; and the inter-day precisions for a relative standard deviation of <10 %. Herein, we describe these two methods for the rapid quantification of protein loaded in DMNs." @default.
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- W3039069481 date "2020-09-01" @default.
- W3039069481 modified "2023-10-03" @default.
- W3039069481 title "Development and evaluation of new methods for protein quantification in dissolving microneedles formulations" @default.
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- W3039069481 doi "https://doi.org/10.1016/j.jpba.2020.113453" @default.
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