Matches in SemOpenAlex for { <https://semopenalex.org/work/W3039137745> ?p ?o ?g. }
- W3039137745 abstract "The relationship between transcription and the 3D chromatin structure is debated. Multiple studies have shown that transcription affects global Cohesin binding and 3D genome structures. However, several other studies have indicated that inhibited transcription does not alter chromatin conformations. We provide the most comprehensive evidence to date to demonstrate that transcription plays a relatively modest role in organizing the local, small-scale chromatin structures in mammalian cells. We show degraded Pol I, Pol II, and Pol III proteins in mESCs cause few or no changes in large-scale 3D chromatin structures, selected RNA polymerases with a high abundance of binding sites or active promoter-associated interactions appear to be relatively more affected after the degradation, transcription inhibition alters local, small loop domains, as indicated by high-resolution chromatin interaction maps, and loops with bound Pol II but without Cohesin or CTCF are identified and found to be largely unchanged after transcription inhibition. Interestingly, Pol II depletion for a longer time significantly affects the chromatin accessibility and Cohesin occupancy, suggesting that RNA polymerases are capable of affecting the 3D genome indirectly. These direct and indirect effects explain the previous inconsistent findings on the influence of transcription inhibition on the 3D genome. We conclude that Pol I, Pol II, and Pol III loss alters local, small-scale chromatin interactions in mammalian cells, suggesting that the 3D chromatin structures are pre-established and relatively stable." @default.
- W3039137745 created "2020-07-10" @default.
- W3039137745 creator A5006596214 @default.
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- W3039137745 creator A5048229809 @default.
- W3039137745 creator A5052994285 @default.
- W3039137745 creator A5058758956 @default.
- W3039137745 creator A5062668398 @default.
- W3039137745 creator A5071548578 @default.
- W3039137745 creator A5080249662 @default.
- W3039137745 date "2020-07-02" @default.
- W3039137745 modified "2023-10-12" @default.
- W3039137745 title "Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II, and Pol III" @default.
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