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- W3039220488 abstract "Abstract Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited. Some pathogenic bacteria have evolved a conserved type III secretion system (T3SS) which allows the delivery of effector proteins into eukaryotic cells. Here, we enhance the T3SS of an avirulent strain of Salmonella typhimurium to reproducibly deliver multiple classes of recombinant proteins into eukaryotic cells. The efficacy of the system is probed with both DARPins and monobodies to functionally inhibit the paradigmatic and largely undruggable RAS signaling pathway. Thus, we develop a bacterial secretion system for potent cytosolic delivery of therapeutic macromolecules." @default.
- W3039220488 created "2020-07-10" @default.
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- W3039220488 date "2020-07-03" @default.
- W3039220488 modified "2023-10-10" @default.
- W3039220488 title "Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol" @default.
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- W3039220488 doi "https://doi.org/10.1038/s42003-020-1072-4" @default.
- W3039220488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7335062" @default.
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- W3039220488 hasPublicationYear "2020" @default.
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