FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3039376113> ?p ?o ?g. }

• W3039376113 abstract "Research on mitochondrial metabolism and respiration are rapidly developing areas, however, efficient and widely accepted methods for studying these in solid tumors are still missing. Here, we developed a new method without isotope tracing to quantitate time dependent mitochondrial citrate efflux in cell lines and human breast cancer samples. In addition, we studied ADP-activated respiration in both of the sample types using selective permeabilization and showed that metabolic activity and respiration are not equally linked. Three times lower amount of mitochondria in scarcely respiring MDA-MB-231 cells convert pyruvate and glutamate into citrate efflux at 20% higher rate than highly respiring MCF-7 mitochondria do. Surprisingly, analysis of 59 human breast cancers revealed the opposite in clinical samples as aggressive breast cancer subtypes, in comparison to less aggressive subtypes, presented with both higher mitochondrial citrate efflux and higher respiration rate. Additionally, comparison of substrate preference (pyruvate or glutamate) for both mitochondrial citrate efflux and respiration in triple negative breast cancers revealed probable causes for high glutamine dependence in this subtype and reasons why some of these tumors are able to overcome glutaminase inhibition. Our research concludes that the two widely used breast cancer cell lines fail to replicate mitochondrial function as seen in respective human samples. And finally, the easy method described here, where time dependent small molecule metabolism and ADP-activated respiration in solid human cancers are analyzed together, can increase success of translational research and ultimately benefit patients with cancer." @default.
• W3039376113 created "2020-07-10" @default.
• W3039376113 creator A5000622522 @default.
• W3039376113 creator A5003926152 @default.
• W3039376113 creator A5023098135 @default.
• W3039376113 creator A5033532576 @default.
• W3039376113 creator A5034855820 @default.
• W3039376113 creator A5038214202 @default.
• W3039376113 creator A5039072406 @default.
• W3039376113 creator A5047423291 @default.
• W3039376113 creator A5055777045 @default.
• W3039376113 creator A5065029405 @default.
• W3039376113 creator A5084129486 @default.
• W3039376113 date "2020-06-30" @default.
• W3039376113 modified "2023-10-17" @default.
• W3039376113 title "Metabolic and OXPHOS Activities Quantified by Temporal ex vivo Analysis Display Patient-Specific Metabolic Vulnerabilities in Human Breast Cancers" @default.
• W3039376113 cites W1522325269 @default.
• W3039376113 cites W1542197083 @default.
• W3039376113 cites W1544928020 @default.
• W3039376113 cites W1599961267 @default.
• W3039376113 cites W1645984643 @default.
• W3039376113 cites W1916910865 @default.
• W3039376113 cites W1929533080 @default.
• W3039376113 cites W1943122884 @default.
• W3039376113 cites W1967186439 @default.
• W3039376113 cites W1971303004 @default.
• W3039376113 cites W1979119227 @default.
• W3039376113 cites W1996365670 @default.
• W3039376113 cites W2004092450 @default.
• W3039376113 cites W2015859860 @default.
• W3039376113 cites W2028114269 @default.
• W3039376113 cites W2044726034 @default.
• W3039376113 cites W2063767304 @default.
• W3039376113 cites W2068971637 @default.
• W3039376113 cites W2072451938 @default.
• W3039376113 cites W2086546138 @default.
• W3039376113 cites W2095987251 @default.
• W3039376113 cites W2097483643 @default.
• W3039376113 cites W2116516144 @default.
• W3039376113 cites W2127057139 @default.
• W3039376113 cites W2132117833 @default.
• W3039376113 cites W2138709778 @default.
• W3039376113 cites W2143408204 @default.
• W3039376113 cites W2150342902 @default.
• W3039376113 cites W2223535266 @default.
• W3039376113 cites W2234115940 @default.
• W3039376113 cites W2258066491 @default.
• W3039376113 cites W2312291090 @default.
• W3039376113 cites W2402636472 @default.
• W3039376113 cites W2516006356 @default.
• W3039376113 cites W2517861725 @default.
• W3039376113 cites W2521748409 @default.
• W3039376113 cites W2567063818 @default.
• W3039376113 cites W2588856093 @default.
• W3039376113 cites W2608433929 @default.
• W3039376113 cites W2637282435 @default.
• W3039376113 cites W2809281987 @default.
• W3039376113 cites W2810450068 @default.
• W3039376113 cites W2924911015 @default.
• W3039376113 cites W2943489129 @default.
• W3039376113 cites W2985764787 @default.
• W3039376113 cites W2988303912 @default.
• W3039376113 cites W3003593776 @default.
• W3039376113 cites W4252205273 @default.
• W3039376113 cites W954944586 @default.
• W3039376113 doi "https://doi.org/10.3389/fonc.2020.01053" @default.
• W3039376113 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7339107" @default.
• W3039376113 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32695682" @default.
• W3039376113 hasPublicationYear "2020" @default.
• W3039376113 type Work @default.
• W3039376113 sameAs 3039376113 @default.
• W3039376113 citedByCount "4" @default.
• W3039376113 countsByYear W30393761132021 @default.
• W3039376113 countsByYear W30393761132022 @default.
• W3039376113 countsByYear W30393761132023 @default.
• W3039376113 crossrefType "journal-article" @default.
• W3039376113 hasAuthorship W3039376113A5000622522 @default.
• W3039376113 hasAuthorship W3039376113A5003926152 @default.
• W3039376113 hasAuthorship W3039376113A5023098135 @default.
• W3039376113 hasAuthorship W3039376113A5033532576 @default.
• W3039376113 hasAuthorship W3039376113A5034855820 @default.
• W3039376113 hasAuthorship W3039376113A5038214202 @default.
• W3039376113 hasAuthorship W3039376113A5039072406 @default.
• W3039376113 hasAuthorship W3039376113A5047423291 @default.
• W3039376113 hasAuthorship W3039376113A5055777045 @default.
• W3039376113 hasAuthorship W3039376113A5065029405 @default.
• W3039376113 hasAuthorship W3039376113A5084129486 @default.
• W3039376113 hasBestOaLocation W30393761131 @default.
• W3039376113 hasConcept C105702510 @default.
• W3039376113 hasConcept C111133373 @default.
• W3039376113 hasConcept C121608353 @default.
• W3039376113 hasConcept C170493617 @default.
• W3039376113 hasConcept C181199279 @default.
• W3039376113 hasConcept C182215343 @default.
• W3039376113 hasConcept C2779349466 @default.
• W3039376113 hasConcept C28859421 @default.
• W3039376113 hasConcept C502942594 @default.
• W3039376113 hasConcept C515207424 @default.
• W3039376113 hasConcept C530470458 @default.
• W3039376113 hasConcept C54355233 @default.

• next