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- W3039477630 abstract "Abstract Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans , using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample. Graphical abstract" @default.
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- W3039477630 date "2020-07-06" @default.
- W3039477630 modified "2023-10-16" @default.
- W3039477630 title "Metabolomics and lipidomics in C. elegans using a single sample preparation" @default.
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- W3039477630 doi "https://doi.org/10.1101/2020.07.06.190017" @default.
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