Matches in SemOpenAlex for { <https://semopenalex.org/work/W3040133544> ?p ?o ?g. }
- W3040133544 abstract "AbstractPlant systems have now gained much attention as they provide low risk of pathogen contamination and cost of production, appropriate post-translational modification, and multimeric assembly capability compared to the mammalian and bacterial hosts. In this study, we aimed to produce single-chain fragment antibody (scFv) encoding anti-Toxoplasma proteins, TP60 in Nicotiana tabacum cv. SR1. Leaf explants of N. tabacum were infected with Agrobacterium tumefaciens strain LBA4404 harbouring binary vector pCAMBIA1304 containing TP60 gene. Putative transformants were confirmed through GUS and GFP qualitative assays. Bands observed at the predicted size of 914 bp confirmed the presence of TP60 transgene and the transgene was stably integrated in both T0 and T1 tobacco genome. FV12-6 transgenic line produced the highest mRNA expression (7-fold) correlated to the highest accumulation of anti-Toxoplasma recombinant scFv antibody (0.52 % of the total soluble protein), followed by FV16-10 (0.25 %), FV17-7 and FV3-11 (0.18 %). The expression of TP60 transgene did not affect the growth of the transgenic plants and the segregation analysis of FV3, FV12, and FV16 in T1 generation confirmed the transgene integration within a single locus according to 3:1 Mendelian’s law. These findings indicated the possibility of using plants as a bio-factory for recombinant protein production. AbstrakSistem tumbuhan kini telah mendapat banyak perhatian kerana mereka menyediakan risiko yang rendah terhadap pencemaran patogen dan kos pengeluaran, pengubahsuaian pasca-protein translasi yang sesuai, dan keupayaan penggabungan multimerik berbanding dengan tentera mamalia dan bakteria. Dalam kajian ini, kami berhasrat untuk menghasilkan antibodi serasi rantaian tunggal (scFv) yang mengekodkan protein anti-Toxoplasma, TP60 di Nicotiana tabacum cv. SR1. Eksplan daun N. tabacum telah dijangkiti oleh Agrobacterium tumefaciens strain LBA4404 yang menyimpan vektor dedua pCAMBIA1304 yang mengandungi gen TP60. Transforman putative disahkan melalui ujian kualitatif GUS dan GFP. Band-band yang diperhatikan pada saiz ramalan 914 bp mengesahkan kehadiran TP60 transgen dan transgen tersebut telah diintegrasi secara stabil dalam genom tembakau T0 dan T1. Individu transgenik FV12-6 menghasilkan ekspresi mRNA tertinggi (7 kali ganda) berkait dengan pengumpulan tertinggi antibodi rekombinan scFv anti-Toxoplasma (0.52% daripada jumlah protein larut), diikuti oleh FV16-10 (0.25%), FV17-7 dan FV3-11 (0.18%). Ungkapan transgenik TP60 tidak menjejaskan pertumbuhan tumbuhan transgenik dan analisis pemisahan FV3, FV12, dan FV16 dalam generasi T1 mengesahkan integrasi transgene dalam satu lokus mengikut 3: 1 undang-undang Mendelian. Penemuan ini menunjukkan kemungkinan menggunakan tumbuhan sebagai kilang bio untuk pengeluaran protein rekombinan. KeywordsAgrobacterium-mediated transformation, plant molecular farming, recombinant protein, single-chain fragment antibody (scFv), tobacco, toxoplasmosis" @default.
- W3040133544 created "2020-07-10" @default.
- W3040133544 creator A5007622097 @default.
- W3040133544 creator A5013000235 @default.
- W3040133544 creator A5036576257 @default.
- W3040133544 creator A5037451464 @default.
- W3040133544 creator A5088393943 @default.
- W3040133544 date "2020-06-30" @default.
- W3040133544 modified "2023-09-23" @default.
- W3040133544 title "NICOTIANA TABACUM AS A POTENTIAL PLATFORM FOR THE PRODUCTION OF RECOMBINANT ANTI-TOXOPLASMA SINGLE-CHAIN VARIABLE FRAGMENT (scFv) ANTIBODY" @default.
- W3040133544 cites W1970195260 @default.
- W3040133544 cites W1981934433 @default.
- W3040133544 cites W1983880486 @default.
- W3040133544 cites W1985690959 @default.
- W3040133544 cites W1987274357 @default.
- W3040133544 cites W1987461381 @default.
- W3040133544 cites W1988697531 @default.
- W3040133544 cites W1989901382 @default.
- W3040133544 cites W1990382626 @default.
- W3040133544 cites W1992653134 @default.
- W3040133544 cites W1998624620 @default.
- W3040133544 cites W2010962189 @default.
- W3040133544 cites W2014158928 @default.
- W3040133544 cites W2015642880 @default.
- W3040133544 cites W2023403405 @default.
- W3040133544 cites W2024556021 @default.
- W3040133544 cites W2026280370 @default.
- W3040133544 cites W2046628664 @default.
- W3040133544 cites W2063678126 @default.
- W3040133544 cites W2064412887 @default.
- W3040133544 cites W2066755382 @default.
- W3040133544 cites W2067459620 @default.
- W3040133544 cites W2069221009 @default.
- W3040133544 cites W2080205209 @default.
- W3040133544 cites W2094812192 @default.
- W3040133544 cites W2103271120 @default.
- W3040133544 cites W2107277218 @default.
- W3040133544 cites W2115251295 @default.
- W3040133544 cites W2128070038 @default.
- W3040133544 cites W2128635872 @default.
- W3040133544 cites W2131025240 @default.
- W3040133544 cites W2131988430 @default.
- W3040133544 cites W2147376578 @default.
- W3040133544 cites W2148580010 @default.
- W3040133544 cites W2155611211 @default.
- W3040133544 cites W2158278323 @default.
- W3040133544 cites W2162019280 @default.
- W3040133544 cites W2168420558 @default.
- W3040133544 cites W2189310977 @default.
- W3040133544 cites W2191486753 @default.
- W3040133544 cites W2258416396 @default.
- W3040133544 cites W2289360724 @default.
- W3040133544 cites W2473939429 @default.
- W3040133544 cites W2559934200 @default.
- W3040133544 cites W2560347605 @default.
- W3040133544 cites W2564250768 @default.
- W3040133544 cites W2591809707 @default.
- W3040133544 cites W2733848854 @default.
- W3040133544 cites W2905300738 @default.
- W3040133544 cites W2912655637 @default.
- W3040133544 cites W2912835684 @default.
- W3040133544 cites W2944271242 @default.
- W3040133544 cites W2962987461 @default.
- W3040133544 cites W2964828653 @default.
- W3040133544 cites W2972026792 @default.
- W3040133544 doi "https://doi.org/10.22452/mjs.vol39no2.1" @default.
- W3040133544 hasPublicationYear "2020" @default.
- W3040133544 type Work @default.
- W3040133544 sameAs 3040133544 @default.
- W3040133544 citedByCount "0" @default.
- W3040133544 crossrefType "journal-article" @default.
- W3040133544 hasAuthorship W3040133544A5007622097 @default.
- W3040133544 hasAuthorship W3040133544A5013000235 @default.
- W3040133544 hasAuthorship W3040133544A5036576257 @default.
- W3040133544 hasAuthorship W3040133544A5037451464 @default.
- W3040133544 hasAuthorship W3040133544A5088393943 @default.
- W3040133544 hasBestOaLocation W30401335441 @default.
- W3040133544 hasConcept C102230213 @default.
- W3040133544 hasConcept C104317684 @default.
- W3040133544 hasConcept C122172344 @default.
- W3040133544 hasConcept C153911025 @default.
- W3040133544 hasConcept C2776162087 @default.
- W3040133544 hasConcept C2776967038 @default.
- W3040133544 hasConcept C2779656422 @default.
- W3040133544 hasConcept C2781205006 @default.
- W3040133544 hasConcept C40767141 @default.
- W3040133544 hasConcept C54355233 @default.
- W3040133544 hasConcept C86803240 @default.
- W3040133544 hasConceptScore W3040133544C102230213 @default.
- W3040133544 hasConceptScore W3040133544C104317684 @default.
- W3040133544 hasConceptScore W3040133544C122172344 @default.
- W3040133544 hasConceptScore W3040133544C153911025 @default.
- W3040133544 hasConceptScore W3040133544C2776162087 @default.
- W3040133544 hasConceptScore W3040133544C2776967038 @default.
- W3040133544 hasConceptScore W3040133544C2779656422 @default.
- W3040133544 hasConceptScore W3040133544C2781205006 @default.
- W3040133544 hasConceptScore W3040133544C40767141 @default.
- W3040133544 hasConceptScore W3040133544C54355233 @default.
- W3040133544 hasConceptScore W3040133544C86803240 @default.
- W3040133544 hasLocation W30401335441 @default.