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- W3040208566 abstract "Abstract Effective engineering approaches for cartilage regeneration involve a combination of cells and biomaterial scaffolds. Multipotent mesenchymal stem cells (MSCs) are important sources for cartilage regeneration. Atelocollagen provides a suitable substrate for MSC attachment and enhancing chondrogenic differentiation. Here, we assessed the chondrogenic potential of adipose tissue derived human MSCs (hMSCs) mixed with atelocollagen gel. We observed cell attachment, viability, and microstructures by electron microscopy over 21 days. The levels of Sox9, type II collagen, aggrecan, type I collagen, Runx2, type X collagen, ALP, Osterix, and MMP13 were measured by RT-qPCR. Cartilage matrix-related proteins were assessed by enzyme-linked immunosorbent assay (ELISA), histology, and immunohistochemistry. hMSCs of all groups exhibited well-maintained cell survival, distribution and morphology. Abundant type II collagen fibers developed on day 21; while Sox9 , type II collagen, and aggrecan expression increased over time in the atelocollagen group. However, type I collagen, RUNX2 , type X collagen ( CoL10A1 ), Osterix , and ALP were not expressed. These results corroborated the protein expression detected by ELISA. Further, histological analysis revealed lacunae-like structures, while staining demonstrated glycosaminoglycan accumulation. Cumulatively, these results indicate that atelocollagen scaffolds improve hMSC chondrogenic differentiation and are a potential approach for cartilage regeneration." @default.
- W3040208566 created "2020-07-10" @default.
- W3040208566 creator A5016177328 @default.
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- W3040208566 date "2020-06-30" @default.
- W3040208566 modified "2023-10-16" @default.
- W3040208566 title "Atelocollagen promotes chondrogenic differentiation of human adipose-derived mesenchymal stem cells" @default.
- W3040208566 cites W1485073457 @default.
- W3040208566 cites W1580844957 @default.
- W3040208566 cites W1594860261 @default.
- W3040208566 cites W1693095538 @default.
- W3040208566 cites W172058065 @default.
- W3040208566 cites W1964502746 @default.
- W3040208566 cites W1969230575 @default.
- W3040208566 cites W1970821370 @default.
- W3040208566 cites W1974207011 @default.
- W3040208566 cites W1976730402 @default.
- W3040208566 cites W1984920924 @default.
- W3040208566 cites W1988904745 @default.
- W3040208566 cites W1989959192 @default.
- W3040208566 cites W1990419270 @default.
- W3040208566 cites W1998024767 @default.
- W3040208566 cites W2000463788 @default.
- W3040208566 cites W2011038175 @default.
- W3040208566 cites W2013767200 @default.
- W3040208566 cites W2018028722 @default.
- W3040208566 cites W2021331319 @default.
- W3040208566 cites W2026763399 @default.
- W3040208566 cites W2027600661 @default.
- W3040208566 cites W2027844006 @default.
- W3040208566 cites W2033889139 @default.
- W3040208566 cites W2036362817 @default.
- W3040208566 cites W2049002990 @default.
- W3040208566 cites W2049157134 @default.
- W3040208566 cites W2052841211 @default.
- W3040208566 cites W2054141235 @default.
- W3040208566 cites W2054657065 @default.
- W3040208566 cites W2064366726 @default.
- W3040208566 cites W2065584680 @default.
- W3040208566 cites W2067600839 @default.
- W3040208566 cites W2069377486 @default.
- W3040208566 cites W2069611045 @default.
- W3040208566 cites W2074355620 @default.
- W3040208566 cites W2076342334 @default.
- W3040208566 cites W2076440518 @default.
- W3040208566 cites W2076923477 @default.
- W3040208566 cites W2086013207 @default.
- W3040208566 cites W2087032263 @default.
- W3040208566 cites W2088396421 @default.
- W3040208566 cites W2091695131 @default.
- W3040208566 cites W2092769775 @default.
- W3040208566 cites W2093800167 @default.
- W3040208566 cites W2096467350 @default.
- W3040208566 cites W2110052568 @default.
- W3040208566 cites W2114804734 @default.
- W3040208566 cites W2118927304 @default.
- W3040208566 cites W2139867678 @default.
- W3040208566 cites W2141588974 @default.
- W3040208566 cites W2141598500 @default.
- W3040208566 cites W2146679173 @default.
- W3040208566 cites W2147083273 @default.
- W3040208566 cites W2153949260 @default.
- W3040208566 cites W2189647639 @default.
- W3040208566 cites W2260087333 @default.
- W3040208566 cites W2260581824 @default.
- W3040208566 cites W2276839800 @default.
- W3040208566 cites W2279353265 @default.
- W3040208566 cites W2344794523 @default.
- W3040208566 cites W2345127954 @default.
- W3040208566 cites W2401989736 @default.
- W3040208566 cites W2442140678 @default.
- W3040208566 cites W2464922076 @default.
- W3040208566 cites W2475212181 @default.
- W3040208566 cites W2513551816 @default.
- W3040208566 cites W2562227412 @default.
- W3040208566 cites W2593575022 @default.
- W3040208566 cites W2604459212 @default.
- W3040208566 cites W2624630779 @default.
- W3040208566 cites W2767897373 @default.
- W3040208566 cites W2790100607 @default.
- W3040208566 cites W2803286735 @default.
- W3040208566 cites W2899207030 @default.
- W3040208566 cites W2909103952 @default.
- W3040208566 cites W2923422505 @default.
- W3040208566 cites W3006679018 @default.
- W3040208566 cites W3008068121 @default.
- W3040208566 cites W38879183 @default.
- W3040208566 doi "https://doi.org/10.1038/s41598-020-67836-3" @default.
- W3040208566 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7327030" @default.
- W3040208566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32606308" @default.
- W3040208566 hasPublicationYear "2020" @default.
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