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- W3040378617 abstract "Meiotic recombination is initiated by formation of DNA double-strand breaks (DSBs). This involves a protein complex that includes in plants the two similar proteins, SPO11-1 and SPO11-2. We analysed the sequences of SPO11-2 in hexaploid bread wheat (Triticum aestivum), as well as in its diploid and tetraploid progenitors. We investigated its role during meiosis using single, double and triple mutants. The three homoeologous SPO11-2 copies of hexaploid wheat exhibit high nucleotide and amino acid similarities with those of the diploids, tetraploids and Arabidopsis. Interestingly, however, two nucleotides deleted in exon-2 of the A copy lead to a premature stop codon and suggest that it encodes a non-functional protein. Remarkably, the mutation was absent from the diploid A-relative Triticum urartu, but present in the tetraploid Triticum dicoccoides and in different wheat cultivars indicating that the mutation occurred after the first polyploidy event and has since been conserved. We further show that triple mutants with all three copies (A, B, D) inactivated are sterile. Cytological analyses of these mutants show synapsis defects, accompanied by severe reductions in bivalent formation and numbers of DMC1 foci, thus confirming the essential role of TaSPO11-2 in meiotic recombination in wheat. In accordance with its 2-nucleotide deletion in exon-2, double mutants for which only the A copy remained are also sterile. Notwithstanding, some DMC1 foci remain visible in this mutant, suggesting a residual activity of the A copy, albeit not sufficient to restore fertility." @default.
- W3040378617 created "2020-07-10" @default.
- W3040378617 creator A5006100803 @default.
- W3040378617 creator A5021766287 @default.
- W3040378617 creator A5023385262 @default.
- W3040378617 creator A5044453166 @default.
- W3040378617 creator A5073595428 @default.
- W3040378617 creator A5074521742 @default.
- W3040378617 date "2020-07-28" @default.
- W3040378617 modified "2023-09-30" @default.
- W3040378617 title "SPO11.2 is essential for programmed double‐strand break formation during meiosis in bread wheat (<i>Triticum aestivum</i>L.)" @default.
- W3040378617 cites W102831228 @default.
- W3040378617 cites W109338624 @default.
- W3040378617 cites W1505792072 @default.
- W3040378617 cites W1510396537 @default.
- W3040378617 cites W1521248195 @default.
- W3040378617 cites W1629989746 @default.
- W3040378617 cites W1784284774 @default.
- W3040378617 cites W1798981132 @default.
- W3040378617 cites W1876047377 @default.
- W3040378617 cites W1969575610 @default.
- W3040378617 cites W1971660736 @default.
- W3040378617 cites W1973538970 @default.
- W3040378617 cites W1980666758 @default.
- W3040378617 cites W1990699200 @default.
- W3040378617 cites W1993968374 @default.
- W3040378617 cites W1999673637 @default.
- W3040378617 cites W2002454928 @default.
- W3040378617 cites W2011768178 @default.
- W3040378617 cites W2014814201 @default.
- W3040378617 cites W2016505307 @default.
- W3040378617 cites W2023488322 @default.
- W3040378617 cites W2023682312 @default.
- W3040378617 cites W2031912474 @default.
- W3040378617 cites W2037382834 @default.
- W3040378617 cites W2037623448 @default.
- W3040378617 cites W2040350740 @default.
- W3040378617 cites W2044575603 @default.
- W3040378617 cites W2047106949 @default.
- W3040378617 cites W2047600884 @default.
- W3040378617 cites W2048067282 @default.
- W3040378617 cites W2049355822 @default.
- W3040378617 cites W2050081633 @default.
- W3040378617 cites W2057652201 @default.
- W3040378617 cites W2064972299 @default.
- W3040378617 cites W2068571698 @default.
- W3040378617 cites W2070617930 @default.
- W3040378617 cites W2074329485 @default.
- W3040378617 cites W2074637295 @default.
- W3040378617 cites W2078101442 @default.
- W3040378617 cites W2078648183 @default.
- W3040378617 cites W2079417931 @default.
- W3040378617 cites W2083938750 @default.
- W3040378617 cites W2102790750 @default.
- W3040378617 cites W2108871842 @default.
- W3040378617 cites W2109322710 @default.
- W3040378617 cites W2109494124 @default.
- W3040378617 cites W2116122104 @default.
- W3040378617 cites W2118386667 @default.
- W3040378617 cites W2119734903 @default.
- W3040378617 cites W2120099260 @default.
- W3040378617 cites W2126822266 @default.
- W3040378617 cites W2127774996 @default.
- W3040378617 cites W2128059090 @default.
- W3040378617 cites W2128695197 @default.
- W3040378617 cites W2129458434 @default.
- W3040378617 cites W2133993902 @default.
- W3040378617 cites W2139380885 @default.
- W3040378617 cites W2141282191 @default.
- W3040378617 cites W2143719385 @default.
- W3040378617 cites W2149819432 @default.
- W3040378617 cites W2156273926 @default.
- W3040378617 cites W2157350189 @default.
- W3040378617 cites W2158714788 @default.
- W3040378617 cites W2159189347 @default.
- W3040378617 cites W2161758325 @default.
- W3040378617 cites W2166223300 @default.
- W3040378617 cites W2170806459 @default.
- W3040378617 cites W2275186331 @default.
- W3040378617 cites W2290357221 @default.
- W3040378617 cites W2302254328 @default.
- W3040378617 cites W2383134261 @default.
- W3040378617 cites W2466168590 @default.
- W3040378617 cites W2475820846 @default.
- W3040378617 cites W2492988610 @default.
- W3040378617 cites W2549029187 @default.
- W3040378617 cites W2603792353 @default.
- W3040378617 cites W2726347871 @default.
- W3040378617 cites W2736096608 @default.
- W3040378617 cites W2770565810 @default.
- W3040378617 cites W2803066701 @default.
- W3040378617 cites W2886541918 @default.
- W3040378617 cites W2894854298 @default.
- W3040378617 cites W2949836654 @default.
- W3040378617 cites W2951518471 @default.
- W3040378617 cites W2980663157 @default.
- W3040378617 cites W2987292751 @default.
- W3040378617 cites W3080074763 @default.