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• W3040431345 endingPage "100242" @default.
• W3040431345 startingPage "100242" @default.
• W3040431345 abstract "Extensive irrigation and unscrupulous use of chemical fertilizers which have led to increased soil salinity (sodicity), are threatening sustainability of agriculture. Plants challenged by soil salinity, tend to adapt by way of altered gene expression. miRNA mediated post transcriptional regulation of gene expression is one of the principal modes of adaptation to stress. The present study aims to gain an insight to the conserved and potential novel niger miRNAs associated with salinity stress responses, with the help of next generation sequencing followed by qRT-PCR validation. 212 and 203, conserved miRNAs, respectively, from control and (300 mM Nacl) salinity stressed libraries of an oil producing plant, niger, have been identified by next generation sequencing (NGS). Six and 16 potential novel miRNAs, respectively from control and stressed niger have been predicted from these libraries. Evaluation of these miRNAs by poly(A)-based qRT-PCR (Quantitative real-time PCR) showed up-regulation of 6 miRNAs; miR166a, miR169e-5p, miR156, miR6173, miR6478 and miR166U and down-regulation of 4 miRNAs; miR166e-3p, miR156a, miR159b and miR169h. Target prediction employing psRNA Target tool indicated 24,648 targets for conserved miRNAs and 1402 targets for potential novel miRNAs of both the libraries. Using gene ontology of predicted targets of the identified miRNAs, provisional network of post transcriptional regulation, contributing to stress response in niger is proposed. Our work presented a rich source for further characterization miRNAs in response to other types of abiotic stress." @default.
• W3040431345 created "2020-07-10" @default.
• W3040431345 creator A5064452324 @default.
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• W3040431345 date "2020-09-01" @default.
• W3040431345 modified "2023-10-18" @default.
• W3040431345 title "Genomic identification of salt induced microRNAs in niger (Guizotia abyssinica Cass.)" @default.
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• W3040431345 doi "https://doi.org/10.1016/j.plgene.2020.100242" @default.
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