FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3040587466> ?p ?o ?g. }

• W3040587466 endingPage "111948" @default.
• W3040587466 startingPage "111948" @default.
• W3040587466 abstract "The harmful effects of low energy UVA photons (315–400 nm) are associated with the massive production of reactive oxygen species resulting in oxidative stress. In response to oxidative damage, NF-E2-related factor 2 (Nrf2) is translocated to the nucleus and drives the expression of detoxication and antioxidant enzymes. UVA's effect on Nrf2 has been quite well characterised in dermal fibroblasts. However, there is a dearth of such information for keratinocytes. This study aimed to evaluate and compare the effect of UVA radiation on the Nrf2 pathway and oxidative stress related proteins in primary human dermal fibroblasts (NHDF), epidermal keratinocytes (NHEK) and human keratinocyte cell line HaCaT. NHDF were exposed to doses of 2.5–7.5 J/cm2, NHEK and HaCaT to 10–20 J/cm2 using a solar simulator. Effects on Nrf2 translocation were evaluated after 1, 3 and 6 h and Nrf2-controlled proteins (heme oxygenase 1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), glutathione reductase (GSR), glutathione-S-transferase (GST), interleukine-6 (IL-6), and matrix metalloproteinases (MMP-1, MMP-2)) after 3, 6 and 24 h. The results showed the fastest Nrf2 translocation was in UVA-irradiated HaCaT (1 h), persisting until the subsequent time interval (3 h), while in primary keratinocytes the effect of radiation was minimal. In NHDF, UVA-stimulated Nrf2 translocation was conspicuous 3 h after UVA treatment. In NHDF, most of the studied proteins (NQO1, HO-1, GSR, GSTM1 and MMP-1) showed the highest level 24 h after UVA exposure, except for MMP-2 and IL-6 which had their highest level at a shorter time incubation interval (3 h). In NHEK, NQO1, HO-1 and GST were increased 6 h after UVA exposure, GSR and MMP-2 level was slightly below or above the control level, and MMP-1 and IL-6 increased at shorter time intervals. When comparing NHEK and HaCaT, these cells displayed contrary responses in most of the Nrf2-controlled proteins. Thus, primary keratinocytes cannot be replaced with HaCaT when studying cell signalling such as the Nrf2 driven pathway and Nrf2-controlled proteins." @default.
• W3040587466 created "2020-07-10" @default.
• W3040587466 creator A5032287413 @default.
• W3040587466 creator A5033786584 @default.
• W3040587466 creator A5042450783 @default.
• W3040587466 creator A5043563539 @default.
• W3040587466 creator A5048640318 @default.
• W3040587466 creator A5057573236 @default.
• W3040587466 creator A5069066705 @default.
• W3040587466 creator A5085310552 @default.
• W3040587466 creator A5091235127 @default.
• W3040587466 date "2020-08-01" @default.
• W3040587466 modified "2023-10-03" @default.
• W3040587466 title "Effect of UVA radiation on the Nrf2 signalling pathway in human skin cells" @default.
• W3040587466 cites W1501578503 @default.
• W3040587466 cites W1641771942 @default.
• W3040587466 cites W1826137220 @default.
• W3040587466 cites W1968131964 @default.
• W3040587466 cites W1969851621 @default.
• W3040587466 cites W1972738625 @default.
• W3040587466 cites W1979813841 @default.
• W3040587466 cites W1983644941 @default.
• W3040587466 cites W1983973679 @default.
• W3040587466 cites W1992356391 @default.
• W3040587466 cites W1994879522 @default.
• W3040587466 cites W2005846423 @default.
• W3040587466 cites W2011493869 @default.
• W3040587466 cites W2011862919 @default.
• W3040587466 cites W2013634364 @default.
• W3040587466 cites W2022017751 @default.
• W3040587466 cites W2022582116 @default.
• W3040587466 cites W2030565582 @default.
• W3040587466 cites W2032935857 @default.
• W3040587466 cites W2033969276 @default.
• W3040587466 cites W2035812835 @default.
• W3040587466 cites W2042442020 @default.
• W3040587466 cites W2044384443 @default.
• W3040587466 cites W2045951593 @default.
• W3040587466 cites W2052373472 @default.
• W3040587466 cites W2053076458 @default.
• W3040587466 cites W2059410642 @default.
• W3040587466 cites W2079764138 @default.
• W3040587466 cites W2107277218 @default.
• W3040587466 cites W2117800935 @default.
• W3040587466 cites W2137263855 @default.
• W3040587466 cites W2143464311 @default.
• W3040587466 cites W2199457629 @default.
• W3040587466 cites W2201309722 @default.
• W3040587466 cites W2273624704 @default.
• W3040587466 cites W2335481162 @default.
• W3040587466 cites W2409710963 @default.
• W3040587466 cites W2413600578 @default.
• W3040587466 cites W2563575197 @default.
• W3040587466 cites W2575135533 @default.
• W3040587466 cites W2586166255 @default.
• W3040587466 cites W2596937407 @default.
• W3040587466 cites W2624249880 @default.
• W3040587466 cites W2780716966 @default.
• W3040587466 cites W2887452211 @default.
• W3040587466 cites W2894471006 @default.
• W3040587466 cites W2895714795 @default.
• W3040587466 cites W2907831572 @default.
• W3040587466 cites W2917823744 @default.
• W3040587466 cites W2927165179 @default.
• W3040587466 cites W360722013 @default.
• W3040587466 doi "https://doi.org/10.1016/j.jphotobiol.2020.111948" @default.
• W3040587466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32679512" @default.
• W3040587466 hasPublicationYear "2020" @default.
• W3040587466 type Work @default.
• W3040587466 sameAs 3040587466 @default.
• W3040587466 citedByCount "24" @default.
• W3040587466 countsByYear W30405874662021 @default.
• W3040587466 countsByYear W30405874662022 @default.
• W3040587466 countsByYear W30405874662023 @default.
• W3040587466 crossrefType "journal-article" @default.
• W3040587466 hasAuthorship W3040587466A5032287413 @default.
• W3040587466 hasAuthorship W3040587466A5033786584 @default.
• W3040587466 hasAuthorship W3040587466A5042450783 @default.
• W3040587466 hasAuthorship W3040587466A5043563539 @default.
• W3040587466 hasAuthorship W3040587466A5048640318 @default.
• W3040587466 hasAuthorship W3040587466A5057573236 @default.
• W3040587466 hasAuthorship W3040587466A5069066705 @default.
• W3040587466 hasAuthorship W3040587466A5085310552 @default.
• W3040587466 hasAuthorship W3040587466A5091235127 @default.
• W3040587466 hasConcept C104317684 @default.
• W3040587466 hasConcept C118995209 @default.
• W3040587466 hasConcept C138626823 @default.
• W3040587466 hasConcept C153911025 @default.
• W3040587466 hasConcept C181199279 @default.
• W3040587466 hasConcept C185592680 @default.
• W3040587466 hasConcept C202751555 @default.
• W3040587466 hasConcept C2776151105 @default.
• W3040587466 hasConcept C2776217839 @default.
• W3040587466 hasConcept C2778004101 @default.
• W3040587466 hasConcept C2779219270 @default.
• W3040587466 hasConcept C48349386 @default.
• W3040587466 hasConcept C538909803 @default.
• W3040587466 hasConcept C55493867 @default.

• next