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- W3040637873 abstract "Abstract Thymidine triphosphate bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore linked to the 5‐methyl group via triazole was synthesized through the CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348‐times) presumably due to interactions in major groove and the fluorescence further increases in the single‐stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by a strong decrease of fluorescence. The nucleotide was used as a light‐up building block in real‐time PCR for detection of SARS‐CoV‐2 virus." @default.
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- W3040637873 date "2020-08-17" @default.
- W3040637873 modified "2023-09-30" @default.
- W3040637873 title "Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions" @default.
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- W3040637873 doi "https://doi.org/10.1002/chem.202003192" @default.
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