FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3040804017> ?p ?o ?g. }

• W3040804017 endingPage "e04405" @default.
• W3040804017 startingPage "e04405" @default.
• W3040804017 abstract "Early diagnosis of SARS-CoV-2 infected patients is essential to control the dynamics of the COVID-19 pandemic. We develop a rapid and accurate one-step multiplex TaqMan probe-based real-time RT-PCR assay, along with a computational tool to systematically analyse the data. Our assay could detect to a limit of 15 copies of SARS-CoV-2 transcripts—based on experiments performed by spiking total human RNA with in vitro synthesized viral transcripts. The assay was evaluated by performing 184 validations for the SARS-CoV-2 Nucleocapsid gene and human RNase P as an internal control reference gene with dilutions ranging from 1-100 ng for human RNA on a cohort of 26 clinical samples. 5 of 26 patients were confirmed to be infected with SARS-CoV-2, while 21 tested negative, consistent with the standards. The accuracy of the assay was found to be 100% sensitive and 100% specific based on the 26 clinical samples that need to be further verified using a large number of clinical samples. In summary, we present a rapid, easy to implement real-time PCR based assay with automated analysis using a novel COVID qPCR Analyzer tool with graphical user interface (GUI) to analyze the raw qRT-PCR data in an unbiased manner at a cost of under $3 per reaction and turnaround time of less than 2h, to enable in-house SARS-CoV-2 testing across laboratories." @default.
• W3040804017 created "2020-07-16" @default.
• W3040804017 creator A5008563502 @default.
• W3040804017 creator A5014644000 @default.
• W3040804017 creator A5026937354 @default.
• W3040804017 creator A5030864351 @default.
• W3040804017 creator A5052311310 @default.
• W3040804017 creator A5056213311 @default.
• W3040804017 creator A5058903725 @default.
• W3040804017 creator A5061808905 @default.
• W3040804017 creator A5068664783 @default.
• W3040804017 creator A5078374483 @default.
• W3040804017 creator A5081498212 @default.
• W3040804017 creator A5082085032 @default.
• W3040804017 creator A5086783488 @default.
• W3040804017 date "2020-07-01" @default.
• W3040804017 modified "2023-10-16" @default.
• W3040804017 title "A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2" @default.
• W3040804017 cites W1568152060 @default.
• W3040804017 cites W1981765089 @default.
• W3040804017 cites W1995972061 @default.
• W3040804017 cites W2056481415 @default.
• W3040804017 cites W2065698478 @default.
• W3040804017 cites W2092394444 @default.
• W3040804017 cites W2103740237 @default.
• W3040804017 cites W2160378127 @default.
• W3040804017 cites W2339921413 @default.
• W3040804017 cites W2559258718 @default.
• W3040804017 cites W2591117466 @default.
• W3040804017 cites W2979487934 @default.
• W3040804017 cites W3001195213 @default.
• W3040804017 cites W3001897055 @default.
• W3040804017 cites W3003637715 @default.
• W3040804017 cites W3004318991 @default.
• W3040804017 cites W3008827533 @default.
• W3040804017 cites W3009219040 @default.
• W3040804017 cites W3011127849 @default.
• W3040804017 cites W3012510801 @default.
• W3040804017 cites W3012991084 @default.
• W3040804017 cites W3014222358 @default.
• W3040804017 cites W3014910618 @default.
• W3040804017 cites W3015040045 @default.
• W3040804017 cites W3017152347 @default.
• W3040804017 cites W3017281110 @default.
• W3040804017 cites W3017334101 @default.
• W3040804017 doi "https://doi.org/10.1016/j.heliyon.2020.e04405" @default.
• W3040804017 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7341355" @default.
• W3040804017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32665985" @default.
• W3040804017 hasPublicationYear "2020" @default.
• W3040804017 type Work @default.
• W3040804017 sameAs 3040804017 @default.
• W3040804017 citedByCount "25" @default.
• W3040804017 countsByYear W30408040172020 @default.
• W3040804017 countsByYear W30408040172021 @default.
• W3040804017 countsByYear W30408040172022 @default.
• W3040804017 countsByYear W30408040172023 @default.
• W3040804017 crossrefType "journal-article" @default.
• W3040804017 hasAuthorship W3040804017A5008563502 @default.
• W3040804017 hasAuthorship W3040804017A5014644000 @default.
• W3040804017 hasAuthorship W3040804017A5026937354 @default.
• W3040804017 hasAuthorship W3040804017A5030864351 @default.
• W3040804017 hasAuthorship W3040804017A5052311310 @default.
• W3040804017 hasAuthorship W3040804017A5056213311 @default.
• W3040804017 hasAuthorship W3040804017A5058903725 @default.
• W3040804017 hasAuthorship W3040804017A5061808905 @default.
• W3040804017 hasAuthorship W3040804017A5068664783 @default.
• W3040804017 hasAuthorship W3040804017A5078374483 @default.
• W3040804017 hasAuthorship W3040804017A5081498212 @default.
• W3040804017 hasAuthorship W3040804017A5082085032 @default.
• W3040804017 hasAuthorship W3040804017A5086783488 @default.
• W3040804017 hasBestOaLocation W30408040171 @default.
• W3040804017 hasConcept C104317684 @default.
• W3040804017 hasConcept C111919701 @default.
• W3040804017 hasConcept C142724271 @default.
• W3040804017 hasConcept C143660080 @default.
• W3040804017 hasConcept C153911025 @default.
• W3040804017 hasConcept C159047783 @default.
• W3040804017 hasConcept C176553487 @default.
• W3040804017 hasConcept C184112429 @default.
• W3040804017 hasConcept C204787440 @default.
• W3040804017 hasConcept C2779134260 @default.
• W3040804017 hasConcept C2781188995 @default.
• W3040804017 hasConcept C3007834351 @default.
• W3040804017 hasConcept C3008058167 @default.
• W3040804017 hasConcept C41008148 @default.
• W3040804017 hasConcept C48023723 @default.
• W3040804017 hasConcept C524204448 @default.
• W3040804017 hasConcept C54355233 @default.
• W3040804017 hasConcept C60644358 @default.
• W3040804017 hasConcept C70721500 @default.
• W3040804017 hasConcept C71924100 @default.
• W3040804017 hasConcept C86803240 @default.
• W3040804017 hasConceptScore W3040804017C104317684 @default.
• W3040804017 hasConceptScore W3040804017C111919701 @default.
• W3040804017 hasConceptScore W3040804017C142724271 @default.
• W3040804017 hasConceptScore W3040804017C143660080 @default.
• W3040804017 hasConceptScore W3040804017C153911025 @default.
• W3040804017 hasConceptScore W3040804017C159047783 @default.

• next