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- W3041723379 abstract "Easy, practical, and affordable gene silencing techniques are constantly progressing, and genetic tools such as TALEs, RNAi, and CRISPR/Cas9 have emerged as new techniques for understanding the basic biology and virulence mechanisms of pathogenic organisms, including bacteria. Here, we describe one-step targeted gene silencing in Leptospira biflexa by using plasmids expressing catalytically inactive Streptococcus pyogenes Cas9 (dCas9) and a single-guide RNA (sgRNA) capable of pairing to the coding strand of a desired gene." @default.
- W3041723379 created "2020-07-16" @default.
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- W3041723379 date "2020-01-01" @default.
- W3041723379 modified "2023-10-05" @default.
- W3041723379 title "Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically Inactive Cas9 (dCas9)" @default.
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- W3041723379 doi "https://doi.org/10.1007/978-1-0716-0459-5_10" @default.
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