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- W3042014856 abstract "Abstract MiR-124 is a highly expressed miRNA in the brain and regulates genes involved in neuronal function. We report that miR-124 post-transcriptionally regulates PARP-1. We have identified a highly conserved binding site of miR-124 in the 3′-untranslated region (3′UTR) of Parp-1 mRNA. We demonstrate that miR-124 directly binds to the Parp-1 3′UTR and mutations in the seed sequences abrogate binding between the two RNA molecules. Luciferase reporter assay revealed that miR-124 post-transcriptionally regulates Parp-1 3′UTR activity in a dopaminergic neuronal cell model. Interestingly, the binding region of miR-124 in Parp-1 3′UTR overlapped with the target sequence of miR-125b, another post-transcriptional regulator of Parp-1 . Our results from titration and pull-down studies revealed that miR-124 binds to Parp-1 3′UTR with greater affinity and confers a dominant post-transcriptional inhibition compared to miR-125b. Interestingly, acute or chronic cocaine exposure downregulated miR-124 levels concomitant with upregulation of PARP-1 protein in dopaminergic-like neuronal cells in culture. Levels of miR-124 were also downregulated upon acute or chronic cocaine exposure in the mouse nucleus accumbens (NAc)-a key reward region of brain. Time-course studies revealed that cocaine treatment persistently downregulated miR-124 in NAc. Consistent with this finding, miR-124 expression was also significantly reduced in the NAc of animals conditioned for cocaine place preference. Collectively, these studies identify Parp-1 as a direct target of miR-124 in neuronal cells, establish miR-124 as a cocaine-regulated miRNA in the mouse NAc, and highlight a novel pathway underlying the molecular effects of cocaine." @default.
- W3042014856 created "2020-07-16" @default.
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- W3042014856 date "2020-07-08" @default.
- W3042014856 modified "2023-10-17" @default.
- W3042014856 title "Cocaine-regulated microRNA miR-124 controls poly (ADP-ribose) polymerase-1 expression in neuronal cells" @default.
- W3042014856 cites W1060653271 @default.
- W3042014856 cites W144423133 @default.
- W3042014856 cites W1541012296 @default.
- W3042014856 cites W1601508141 @default.
- W3042014856 cites W1602460289 @default.
- W3042014856 cites W1916491745 @default.
- W3042014856 cites W1965306643 @default.
- W3042014856 cites W1967377184 @default.
- W3042014856 cites W1971295767 @default.
- W3042014856 cites W1978337436 @default.
- W3042014856 cites W1985974546 @default.
- W3042014856 cites W1988163167 @default.
- W3042014856 cites W1991223384 @default.
- W3042014856 cites W1997973656 @default.
- W3042014856 cites W2000670569 @default.
- W3042014856 cites W2000904576 @default.
- W3042014856 cites W2007612962 @default.
- W3042014856 cites W2007794780 @default.
- W3042014856 cites W2008264529 @default.
- W3042014856 cites W2016112105 @default.
- W3042014856 cites W2016390086 @default.
- W3042014856 cites W2016500819 @default.
- W3042014856 cites W2018091664 @default.
- W3042014856 cites W2021643429 @default.
- W3042014856 cites W2022851859 @default.
- W3042014856 cites W2023997559 @default.
- W3042014856 cites W2035657750 @default.
- W3042014856 cites W2038874091 @default.
- W3042014856 cites W2039784627 @default.
- W3042014856 cites W2042161313 @default.
- W3042014856 cites W2050066317 @default.
- W3042014856 cites W2053930149 @default.
- W3042014856 cites W2056285815 @default.
- W3042014856 cites W2060658307 @default.
- W3042014856 cites W2062256108 @default.
- W3042014856 cites W2063413535 @default.
- W3042014856 cites W2064869544 @default.
- W3042014856 cites W2067775919 @default.
- W3042014856 cites W2068910226 @default.
- W3042014856 cites W2069303592 @default.
- W3042014856 cites W2079590230 @default.
- W3042014856 cites W2081822897 @default.
- W3042014856 cites W2082891074 @default.
- W3042014856 cites W2086864207 @default.
- W3042014856 cites W2089226922 @default.
- W3042014856 cites W2092807015 @default.
- W3042014856 cites W2094253263 @default.
- W3042014856 cites W2098571862 @default.
- W3042014856 cites W2107588151 @default.
- W3042014856 cites W2109577325 @default.
- W3042014856 cites W2112325899 @default.
- W3042014856 cites W2117391818 @default.
- W3042014856 cites W2120616189 @default.
- W3042014856 cites W2123907069 @default.
- W3042014856 cites W2138473891 @default.
- W3042014856 cites W2140002469 @default.
- W3042014856 cites W2143292016 @default.
- W3042014856 cites W2145577632 @default.
- W3042014856 cites W2146803828 @default.
- W3042014856 cites W2147372837 @default.
- W3042014856 cites W2149297993 @default.
- W3042014856 cites W2149472465 @default.
- W3042014856 cites W2151213561 @default.
- W3042014856 cites W2154085311 @default.
- W3042014856 cites W2156023352 @default.
- W3042014856 cites W2157016693 @default.
- W3042014856 cites W2161272397 @default.
- W3042014856 cites W2162674813 @default.
- W3042014856 cites W2171023838 @default.
- W3042014856 cites W2171833185 @default.
- W3042014856 cites W2178682482 @default.
- W3042014856 cites W2186413662 @default.
- W3042014856 cites W2315437876 @default.
- W3042014856 cites W2319228377 @default.
- W3042014856 cites W2398660354 @default.
- W3042014856 cites W2399338035 @default.
- W3042014856 cites W2510657589 @default.
- W3042014856 cites W2558561395 @default.
- W3042014856 cites W2588248122 @default.
- W3042014856 cites W2732147874 @default.
- W3042014856 cites W2732346161 @default.
- W3042014856 cites W2743317735 @default.
- W3042014856 cites W2779763156 @default.