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- W3042370943 endingPage "11163" @default.
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- W3042370943 abstract "Rotationally averaged collision cross section (CCS) values for a series of proteins and protein complexes ranging in size from 8.6 to 810 kDa are reported. The CCSs were obtained using a native electrospray ionization drift tube ion mobility-Orbitrap mass spectrometer specifically designed to enhance sensitivity while having high-resolution ion mobility and mass capabilities. Periodic focusing (PF)-drift tube (DT)-ion mobility (IM) provides first-principles determination of the CCS of large biomolecules that can then be used as CCS calibrants. The experimental, first-principles CCS values are compared to previously reported experimentally determined and computationally calculated CCS using projected superposition approximation (PSA), the Ion Mobility Projection Approximation Calculation Tool (IMPACT), and Collidoscope. Experimental CCS values are generally in agreement with previously reported CCSs, with values falling within ∼5.5%. In addition, an ion mobility resolution (CCS centroid divided by CCS fwhm) of ∼60 is obtained for pyruvate kinase (MW ∼ 233 kDa); however, ion mobility resolution for bovine serum albumin (MW ∼ 68 kDa) is less than ∼20, which arises from sample impurities and underscores the importance of sample quality. The high resolution afforded by the ion mobility-Orbitrap mass analyzer provides new opportunities to understand the intricate details of protein complexes such as the impact of post-translational modifications (PTMs), stoichiometry, and conformational changes induced by ligand binding." @default.
- W3042370943 created "2020-07-23" @default.
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- W3042370943 date "2020-07-14" @default.
- W3042370943 modified "2023-10-18" @default.
- W3042370943 title "First-Principles Collision Cross Section Measurements of Large Proteins and Protein Complexes" @default.
- W3042370943 cites W1665864650 @default.
- W3042370943 cites W1866609041 @default.
- W3042370943 cites W1879205842 @default.
- W3042370943 cites W1964832582 @default.
- W3042370943 cites W1971795378 @default.
- W3042370943 cites W1978027005 @default.
- W3042370943 cites W1978235493 @default.
- W3042370943 cites W1980472600 @default.
- W3042370943 cites W1991599052 @default.
- W3042370943 cites W1991832340 @default.
- W3042370943 cites W1992935534 @default.
- W3042370943 cites W2008342614 @default.
- W3042370943 cites W2008753812 @default.
- W3042370943 cites W2011644673 @default.
- W3042370943 cites W2012023524 @default.
- W3042370943 cites W2012625165 @default.
- W3042370943 cites W2014359578 @default.
- W3042370943 cites W2019108962 @default.
- W3042370943 cites W2026183640 @default.
- W3042370943 cites W2030147742 @default.
- W3042370943 cites W2030175020 @default.
- W3042370943 cites W2036734090 @default.
- W3042370943 cites W2044322490 @default.
- W3042370943 cites W2052130353 @default.
- W3042370943 cites W2052902428 @default.
- W3042370943 cites W2053620269 @default.
- W3042370943 cites W2053779854 @default.
- W3042370943 cites W2056504496 @default.
- W3042370943 cites W2056865451 @default.
- W3042370943 cites W2057385367 @default.
- W3042370943 cites W2059304030 @default.
- W3042370943 cites W2059481685 @default.
- W3042370943 cites W2068722030 @default.
- W3042370943 cites W2069429456 @default.
- W3042370943 cites W2069867742 @default.
- W3042370943 cites W2070948666 @default.
- W3042370943 cites W2072404529 @default.
- W3042370943 cites W2073327318 @default.
- W3042370943 cites W2075510752 @default.
- W3042370943 cites W2076763073 @default.
- W3042370943 cites W2078811015 @default.
- W3042370943 cites W2082125986 @default.
- W3042370943 cites W2083449193 @default.
- W3042370943 cites W2083855333 @default.
- W3042370943 cites W2091354138 @default.
- W3042370943 cites W2094495776 @default.
- W3042370943 cites W2100837269 @default.
- W3042370943 cites W2101982331 @default.
- W3042370943 cites W2103647536 @default.
- W3042370943 cites W2122967365 @default.
- W3042370943 cites W2142415115 @default.
- W3042370943 cites W2145270425 @default.
- W3042370943 cites W2146292423 @default.
- W3042370943 cites W2149789639 @default.
- W3042370943 cites W2154119752 @default.
- W3042370943 cites W2156012420 @default.
- W3042370943 cites W2159322173 @default.
- W3042370943 cites W2161139588 @default.
- W3042370943 cites W2170036411 @default.
- W3042370943 cites W2209257594 @default.
- W3042370943 cites W2275613297 @default.
- W3042370943 cites W2302819319 @default.
- W3042370943 cites W2315485810 @default.
- W3042370943 cites W2317233863 @default.
- W3042370943 cites W2320930590 @default.
- W3042370943 cites W2325096159 @default.
- W3042370943 cites W2334348545 @default.
- W3042370943 cites W2335941517 @default.
- W3042370943 cites W2412083062 @default.
- W3042370943 cites W2413828919 @default.
- W3042370943 cites W2489194202 @default.
- W3042370943 cites W2506865782 @default.
- W3042370943 cites W2525032338 @default.
- W3042370943 cites W2558396410 @default.
- W3042370943 cites W2563935489 @default.
- W3042370943 cites W2588017397 @default.
- W3042370943 cites W2599461440 @default.
- W3042370943 cites W2731018035 @default.
- W3042370943 cites W2731879246 @default.