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- W3042422416 abstract "前期研究结果表明，非洲猪瘟病毒（ASFV）MGF 360-9L能显著抑制宿主天然免疫应答，故通过比较、分析ASFV中MGF 360基因序列，进一步研究MGF 360-9L基因结构和功能间的关系。本研究采用生物信息学方法，分析该基因的一级结构并预测该基因的表达蛋白二、三级结构；根据GenBank公布的Georgia 2007/1（FR682468.1）序列，合成MGF 360-9L基因并构建其重组真核表达质粒，Western blot验证该基因表达后，将重组质粒转染至PK-15细胞，经染色后观察其蛋白的亚细胞定位。结果表明，以Ⅱ型Georgia 2007/1基因组中MGF 360-9L基因为参照，其在Ⅱ型ASFV毒株中高度保守，在54株不同基因型毒株间的相似性与ASFV系统进化关系一致，保守序列为3'末端378 bp片段，高级结构以α螺旋为主，核定位序列预测其定位于细胞核内；构建真核表达质粒，成功表达目的蛋白且定位于细胞核内。本研究结果为进一步研究MGF 360-9L基因抑制免疫应答和明确MGF 360基因家族在ASFV的致病机制积累了资料。" @default.
- W3042422416 created "2020-07-23" @default.
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- W3042422416 date "2020-06-25" @default.
- W3042422416 modified "2023-09-27" @default.
- W3042422416 title "非洲猪瘟病毒MGF 360-9L基因序列分析、蛋白结构预测及亚细胞定位" @default.
- W3042422416 doi "https://doi.org/10.11843/j.issn.0366-6964.2020.020" @default.
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