Matches in SemOpenAlex for { <https://semopenalex.org/work/W3042457375> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W3042457375 endingPage "121406" @default.
- W3042457375 startingPage "121406" @default.
- W3042457375 abstract "Colistimethate sodium (CMS) is a widely administrated old-generation prodrug for the treatment of the life-threatening infections caused by multi-resistant Gram-negative bacteria. Until now, the quality control procedure of the CMS commercial products is based on microbiological assays. The aim of the study is the development of a chemical analysis methodology based on liquid chromatography - mass spectrometry (LC-MS) that could be used for the quality control of CMS products. The careful optimization of the LC and QToF-MS parameters was deemed crucial, as CMS is known to be a very complex mixture. Thus, a two stage Design of Experiments (DoE) pipeline has been followed, aiming towards the separation of the mixture components. According to the DoE results, a baseline-resolved chromatogram revealing more than 20 compounds was achieved. The separation was performed using a Waters Acquity BEH C8 column employing gradient elution. The mobile phase consisted of aq. ammonium formate 0.005 M (pH 6) (solvent A) and methanol/acetonitrile 79/21 (v/v) (solvent B). A second optimization experiment for the MS signal was employed in order to achieve maximum sensitivity. The singly charged signals were monitored for the validation in the positive ion mode. The calibration curve range was 50–110 μg mL−1, corresponding to the 80–120% of the nominal CMS amount in the commercial products. Due to the complexity of the CMS chromatograms and the corresponding spectrum of each chromatographic peak, untargeted and targeted approaches were performed employing the MZmine software. Furthermore, apart from the classical univariate statistical analysis, partial least squares regression (PLS-R) model was also employed, as the variables were more than the observations. The developed methodology has been employed to analyze several batches and inconsistences have been discovered." @default.
- W3042457375 created "2020-07-23" @default.
- W3042457375 creator A5046575282 @default.
- W3042457375 creator A5046815019 @default.
- W3042457375 creator A5068439544 @default.
- W3042457375 date "2020-12-01" @default.
- W3042457375 modified "2023-09-24" @default.
- W3042457375 title "Design of experiments guided multivariate calibration for the quantitation of injectable colistimethate sodium by ultra performance liquid chromatography – High resolution mass spectrometry" @default.
- W3042457375 cites W1973414529 @default.
- W3042457375 cites W1986923773 @default.
- W3042457375 cites W1989299822 @default.
- W3042457375 cites W2020717334 @default.
- W3042457375 cites W2078018953 @default.
- W3042457375 cites W2082630355 @default.
- W3042457375 cites W2091488830 @default.
- W3042457375 cites W2120266293 @default.
- W3042457375 cites W2122721018 @default.
- W3042457375 cites W2163015800 @default.
- W3042457375 cites W2198170714 @default.
- W3042457375 cites W2289993069 @default.
- W3042457375 cites W2358053226 @default.
- W3042457375 cites W2483242681 @default.
- W3042457375 cites W2509216003 @default.
- W3042457375 cites W2525874440 @default.
- W3042457375 cites W2535685529 @default.
- W3042457375 cites W2573177864 @default.
- W3042457375 cites W2744329751 @default.
- W3042457375 cites W2900707259 @default.
- W3042457375 doi "https://doi.org/10.1016/j.talanta.2020.121406" @default.
- W3042457375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32928422" @default.
- W3042457375 hasPublicationYear "2020" @default.
- W3042457375 type Work @default.
- W3042457375 sameAs 3042457375 @default.
- W3042457375 citedByCount "5" @default.
- W3042457375 countsByYear W30424573752021 @default.
- W3042457375 countsByYear W30424573752022 @default.
- W3042457375 crossrefType "journal-article" @default.
- W3042457375 hasAuthorship W3042457375A5046575282 @default.
- W3042457375 hasAuthorship W3042457375A5046815019 @default.
- W3042457375 hasAuthorship W3042457375A5068439544 @default.
- W3042457375 hasConcept C105795698 @default.
- W3042457375 hasConcept C113196181 @default.
- W3042457375 hasConcept C119128265 @default.
- W3042457375 hasConcept C138268822 @default.
- W3042457375 hasConcept C147789679 @default.
- W3042457375 hasConcept C154945302 @default.
- W3042457375 hasConcept C162356407 @default.
- W3042457375 hasConcept C165838908 @default.
- W3042457375 hasConcept C179998833 @default.
- W3042457375 hasConcept C185592680 @default.
- W3042457375 hasConcept C187530423 @default.
- W3042457375 hasConcept C204320433 @default.
- W3042457375 hasConcept C23531484 @default.
- W3042457375 hasConcept C2775986502 @default.
- W3042457375 hasConcept C2776507403 @default.
- W3042457375 hasConcept C33923547 @default.
- W3042457375 hasConcept C41008148 @default.
- W3042457375 hasConcept C43617362 @default.
- W3042457375 hasConceptScore W3042457375C105795698 @default.
- W3042457375 hasConceptScore W3042457375C113196181 @default.
- W3042457375 hasConceptScore W3042457375C119128265 @default.
- W3042457375 hasConceptScore W3042457375C138268822 @default.
- W3042457375 hasConceptScore W3042457375C147789679 @default.
- W3042457375 hasConceptScore W3042457375C154945302 @default.
- W3042457375 hasConceptScore W3042457375C162356407 @default.
- W3042457375 hasConceptScore W3042457375C165838908 @default.
- W3042457375 hasConceptScore W3042457375C179998833 @default.
- W3042457375 hasConceptScore W3042457375C185592680 @default.
- W3042457375 hasConceptScore W3042457375C187530423 @default.
- W3042457375 hasConceptScore W3042457375C204320433 @default.
- W3042457375 hasConceptScore W3042457375C23531484 @default.
- W3042457375 hasConceptScore W3042457375C2775986502 @default.
- W3042457375 hasConceptScore W3042457375C2776507403 @default.
- W3042457375 hasConceptScore W3042457375C33923547 @default.
- W3042457375 hasConceptScore W3042457375C41008148 @default.
- W3042457375 hasConceptScore W3042457375C43617362 @default.
- W3042457375 hasFunder F4320338080 @default.
- W3042457375 hasLocation W30424573751 @default.
- W3042457375 hasOpenAccess W3042457375 @default.
- W3042457375 hasPrimaryLocation W30424573751 @default.
- W3042457375 hasRelatedWork W1994975430 @default.
- W3042457375 hasRelatedWork W2033909045 @default.
- W3042457375 hasRelatedWork W2107172911 @default.
- W3042457375 hasRelatedWork W2350477574 @default.
- W3042457375 hasRelatedWork W2385170954 @default.
- W3042457375 hasRelatedWork W2385872101 @default.
- W3042457375 hasRelatedWork W3028740714 @default.
- W3042457375 hasRelatedWork W322279609 @default.
- W3042457375 hasRelatedWork W4285404929 @default.
- W3042457375 hasRelatedWork W2080078481 @default.
- W3042457375 hasVolume "220" @default.
- W3042457375 isParatext "false" @default.
- W3042457375 isRetracted "false" @default.
- W3042457375 magId "3042457375" @default.
- W3042457375 workType "article" @default.